Molecular Survey of the Dissemination of Two blaKPC-Harboring IncFIA Plasmids in New Jersey and New York Hospitals
Molecular Survey of the Dissemination of Two blaKPC-Harboring IncFIA Plasmids in New Jersey and New York Hospitals
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DOI：
10.1128/aac.02749-13
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发表时间：
2014-04-01
影响因子：
4.9
通讯作者：
Kreiswirth, Barry N.
中科院分区：
文献类型：
--
作者：
Chen, Liang;Chavda, Kalyan D.;Kreiswirth, Barry N.
产碳青霉烯酶（KPC）的肺炎克雷伯菌。肺炎菌株已经在世界范围内传播并成为卫生保健设施中的主要威胁。bla（KPC）是质粒携带的KPC基因，其传播可以通过克隆传播和水平转移介导。在这里，我们报告了两个新的bla（KPC-3）-窝藏IncFIA质粒，pBK 30661和pBK 30683的完整核苷酸序列。pBK 30661长74 kb，具有嵌合质粒结构;它与几种其他质粒具有同源性，但缺乏质粒转移所需的质粒转移操纵子（tra）和转移起点（oriT）。pBK 30683是具有共整合质粒结构的接合质粒，包含高度类似于pBK 30661（>99.9%核苷酸同一性）的72-kb元件和携带tra和oriT的额外68-kb元件。设计PCR方案检测来自新泽西和纽约10家医院的临床肠杆菌科分离株中携带bla（KPC）的IncFIA（pBK 30661-like和pBK 30683-like）质粒的分布。在491株K菌中，有20%的菌株携带有KPC的IncFIA质粒。pneumoniae分离株，均携带bla（KPC-3）。pBK 30661样质粒主要在流行序列类型258（ST 258）K中鉴定。pBK 30683样质粒在ST 258和其它肺炎克雷伯氏菌中广泛分布。pneumoniae序列类型和非K.肺炎肠杆菌科物种。这表明克隆传播和水平质粒转移都有助于携带bla（KPC）的IncFIA质粒在我们地区的传播。需要进一步的研究来了解该质粒群在其他卫生保健区域的分布，并破译pBK 30661样和pBK 30683样质粒的起源。
Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae strains have spread worldwide and become a major threat in health care facilities. Transmission of bla(KPC), the plasmid-borne KPC gene, can be mediated by clonal spread and horizontal transfer. Here, we report the complete nucleotide sequences of two novel bla(KPC-3)-harboring IncFIA plasmids, pBK30661 and pBK30683. pBK30661 is 74 kb in length, with a mosaic plasmid structure; it exhibits homologies to several other plasmids but lacks the plasmid transfer operon (tra) and the origin of transfer (oriT) that are required for plasmid transfer. pBK30683 is a conjugative plasmid with a cointegrated plasmid structure, comprising a 72-kb element that highly resembles pBK30661 (>99.9% nucleotide identities) and an extra 68-kb element that harbors tra and oriT. A PCR scheme was designed to detect the distribution of bla(KPC)-harboring IncFIA (pBK30661-like and pBK30683-like) plasmids in a collection of clinical Enterobacteriaceae isolates from 10 hospitals in New Jersey and New York. KPC-harboring IncFIA plasmids were found in 20% of 491 K. pneumoniae isolates, and all carried bla(KPC-3). pBK30661-like plasmids were identified mainly in the epidemic sequence type 258 (ST258) K. pneumoniae clone, while pBK30683-like plasmids were widely distributed in ST258 and other K. pneumoniae sequence types and among non-K. pneumoniae Enterobacteriaceae species. This suggests that both clonal spread and horizontal plasmid transfer contributed to the dissemination of bla(KPC)-harboring IncFIA plasmids in our area. Further studies are needed to understand the distribution of this plasmid group in other health care regions and to decipher the origins of pBK30661-like and pBK30683-like plasmids.