Circatidal gene expression in the mangrove cricket Apteronemobius asahinai
Circatidal gene expression in the mangrove cricket Apteronemobius asahinai
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DOI：
10.1038/s41598-019-40197-2
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发表时间：
2019-03-06
影响因子：
4.6
通讯作者：
Terai, Yohey
中科院分区：
文献类型：
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作者：
Satoh, Aya;Terai, Yohey
红树林蟋蟀Apteronemobius asahinai是红树林地面特有的。它表现出昼夜节律性，在恒定条件下有12.6小时的自发活动期。它的自由活动也有昼夜节律的组成部分;即它在主观夜间比白天更活跃。在这项研究中，我们研究了节奏基因表达恒定黑暗下的RNA测序，以确定基因控制的生物钟。分析每3 h采集的样品，共48 h（每个时间点一只蟋蟀）。我们确定了284个重要的昼夜循环成绩单（周期长度12-15小时）。其中近一半在NCBI nr数据库中被注释了已知基因，包括与代谢过程和分子伴侣相关的酶。具有昼夜节律性的转录本比具有昼夜节律性的转录本少，核心生物钟基因的表达没有表现出明显的节律性。这可能反映了红树林蟋蟀的性质，也可能是由于采样重复的缺乏：昼夜节律周期只有两个周期，没有重复。我们第一次评估了昆虫的节律性转录组，显示昼夜节律活动，我们的研究结果将有助于未来的昼夜节律时钟基因的研究。
The mangrove cricket Apteronemobius asahinai is endemic to mangrove forest floors. It shows circatidal rhythmicity, with a 12.6-h period of locomotor activity under constant conditions. Its free-running activity also has a circadian component; i.e. it is more active during the subjective night than during the day. In this study, we investigated rhythmic gene expression under constant darkness by RNA sequencing to identify genes controlled by the biological clock. Samples collected every 3 h for 48 h were analysed (one cricket per time-point). We identified 284 significant circatidal cycling transcripts (period length 12-15 h). Almost half of them were annotated with known genes in the NCBI nr database, including enzymes related to metabolic processes and molecular chaperones. There were less transcripts with circadian rhythmicity than with circatidal rhythmicity, and the expression of core circadian clock genes did not show significant rhythmicity. This may reflect the nature of the mangrove cricket or may be due to the paucity of the sampling repeats: only two periods for circadian cycle with no replications. We evaluated for the first time the rhythmic transcriptome of an insect that shows circatidal rhythmic activity; our findings will contribute to future studies of circatidal clock genes.