Effect of phosphoramidon on brain tissue angiogenesis in the chronic phase of vasospasm after subarachnoid hemorrhage in the rat.
Effect of phosphoramidon on brain tissue angiogenesis in the chronic phase of vasospasm after subarachnoid hemorrhage in the rat.
复制标题
磷酰胺对大鼠蛛网膜下腔出血后血管痉挛慢性期脑组织血管生成的影响。
DOI：
--
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
J. Jochem
中科院分区：
文献类型：
--
作者：
J. Jośko;S. Hendryk;H. Jędrzejowska‐Szypułka;J. Słowiński;B. Gwóźdź;D. Lange;M. Śnietura;K. Żwirska;J. Jochem
背景
蛛网膜下腔出血（SAH）经常导致长时间的脑血管痉挛，导致内皮细胞缺血和神经元缺氧导致血管病变。出血后血管痉挛可以通过施用磷酰胺来抵消，磷酰胺可阻断负责将大内皮素转化为完全活性的 ET1 肽的内皮素转化酶 (ECE)。本研究的目的是确定 SAH 后慢性血管痉挛对血管生成的影响，以及内皮素 1（血管痉挛的主要致病因素）对此过程的影响。
材料和方法
对雄性 Wistar 大鼠进行了检查。小脑延髓池插管后 7 天，给予血液以诱导 SAH。 ECE抑制剂磷酰胺以40nmol的剂量在50微升脑脊液中施用3次：SAH前20分钟、SAH后60分钟和SAH后24小时。 48小时后取出大脑进行组织学评估。在大脑半球切片（海马背侧部分水平）和脑干切片（脑桥的1/2）中测量血管表面密度。
结论
SAH 后大脑半球的血管生成增加。施用磷酰胺可抑制 SAH 后大脑半球的血管生成。
BACKGROUND
Subarachnoid hemorrhage (SAH) frequently leads to prolonged cerebral vasospasm resulting in vascular pathology due to endothelial cell ischemia and neuronal hypoxia. Posthemorrhagic vasospasm can be counteracted by the administration of phosphoramidon, which blocks the endothelin converting enzyme (ECE) responsible for the conversion of big endothelin into a fully active ET1 peptide. The aim of the study was to determine the effect of chronic vasospasm after SAH on angiogenesis and the effect on this process of endothelin-1, the main causative factor in vasospasm.
MATERIAL AND METHODS
Male Wistar rats were examined. Seven days after cannulation of the cisterna magna, blood was administered to induce SAH. The ECE inhibitor phosphoramidon was administered in a dose of 40 nmol in 50 microl of cerebrospinal fluid three times: 20 min before SAH, 60 min after SAH, and 24 hours after SAH. The brains were removed 48 hours later for histological evaluation. The vascular surface density was measured in cerebral hemisphere sections (at the level of the dorsal part of the hippocampus) and brainstem sections (1/2 of the pons).
CONCLUSION
Increased angiogenesis was observed in the cerebral hemispheres after SAH. The administration of phosphoramidon inhibits angiogenesis in cerebral hemispheres after SAH.