Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae
Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae
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DOI：
10.1126/science.289.5487.2126
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发表时间：
2000-09-22
期刊：
影响因子：
56.9
通讯作者：
Guarente, L
中科院分区：
文献类型：
--
作者：
Lin, SJ;Defossez, PA;Guarente, L
限制热量可以延长多种生物体的寿命。尽管有人建议限制卡路里可能是通过减少呼吸过程中产生的活性氧来起作用的。这种养生法延缓衰老的机制尚不确定。在这里，我们通过生理或遗传手段模拟酵母中的卡路里限制，并显示出寿命的大幅延长。在SIR2（其编码沉默蛋白Sir2p）或NPT1（NAD合成途径中的基因，烟酰胺腺嘌呤二核苷酸的氧化形式）的菌株突变体中未观察到这种延伸。这些发现表明，由卡路里限制诱导的寿命增加需要NAD激活Sir2p。
Calorie restriction extends Life-span in a wide variety of organisms. Although it has been suggested that calorie restriction may work by reducing the levels of reactive oxygen species produced during respiration. the mechanism by which this regimen slows aging is uncertain. Here, we mimicked calorie restriction in yeast by physiological or genetic means and showed a substantial extension in Life-span. This extension was not observed in strains mutant for SIR2 (which encodes the silencing protein Sir2p) or NPT1 (a gene in a pathway in the synthesis of NAD, the oxidized form of nicotinamide adenine dinucleotide), These findings suggest that the increased longevity induced by calorie restriction requires the activation of Sir2p by NAD.