Rotation of endosomes demonstrates coordination of molecular motors during axonal transport.
Rotation of endosomes demonstrates coordination of molecular motors during axonal transport.
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DOI：
10.1126/sciadv.1602170
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发表时间：
2018-03
期刊：
影响因子：
13.6
通讯作者：
Cui B
中科院分区：
文献类型：
--
作者：
Kaplan L;Ierokomos A;Chowdary P;Bryant Z;Cui B
多极显微镜显示轴突内体的旋转动力学与分子马达活动的紧密联系。长距离轴突运输对神经元的维持和功能至关重要。强大的运输是由多个分子马达在一个团队中协调活动来确保的。在轴突运输研究中使用的常规活细胞成像技术通过可视化货物的平移动力学来检测这种活动。然而，平移测量对由运动活动引起的扭矩不敏感。通过使用金纳米棒和多通道偏振显微镜，我们同时测量旋转和平移动力学的数千个轴突运输的内体。我们发现，内体的旋转动力学提供了关于分子马达活动的补充信息，以常规跟踪的平移动力学。旋转动力学与平移动力学相关，特别是在驱动蛋白和动力蛋白介导的运输之间切换后旋转增加的情况下。此外，毫不含糊的测量纳米棒的角度表明，内体包含的纳米棒与微管的方向对齐，这表明配体-受体复合物和微管马达之间的直接机械连接。
Multipolarization microscopy shows tight linkage of the rotational dynamics of axonal endosomes to molecular motor activity. Long-distance axonal transport is critical to the maintenance and function of neurons. Robust transport is ensured by the coordinated activities of multiple molecular motors acting in a team. Conventional live-cell imaging techniques used in axonal transport studies detect this activity by visualizing the translational dynamics of a cargo. However, translational measurements are insensitive to torques induced by motor activities. By using gold nanorods and multichannel polarization microscopy, we simultaneously measure the rotational and translational dynamics for thousands of axonally transported endosomes. We find that the rotational dynamics of an endosome provide complementary information regarding molecular motor activities to the conventionally tracked translational dynamics. Rotational dynamics correlate with translational dynamics, particularly in cases of increased rotation after switches between kinesin- and dynein-mediated transport. Furthermore, unambiguous measurement of nanorod angle shows that endosome-contained nanorods align with the orientation of microtubules, suggesting a direct mechanical linkage between the ligand-receptor complex and the microtubule motors.
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影响因子：
5.3
作者：
Her, Lu-Shiun;Goldstein, Lawrence S. B.
通讯作者：
Goldstein, Lawrence S. B.
影响因子：
64.5
作者：
Galiano MR;Jha S;Ho TS;Zhang C;Ogawa Y;Chang KJ;Stankewich MC;Mohler PJ;Rasband MN
通讯作者：
Rasband MN
DOI：
10.1073/pnas.1107841108
发表时间：
2011-11-22
影响因子：
11.1
作者：
Kunwar, Ambarish;Tripathy, Suvranta K.;Gross, Steven P.
通讯作者：
Gross, Steven P.
DOI：
10.1126/science.1226734
发表时间：
2012-11-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Derr ND;Goodman BS;Jungmann R;Leschziner AE;Shih WM;Reck-Peterson SL
通讯作者：
Reck-Peterson SL
DOI：
10.1083/jcb.201106120
发表时间：
2012-02-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Maday S;Wallace KE;Holzbaur EL
通讯作者：
Holzbaur EL