Systematic analysis of potato acid invertase genes reveals that a cold-responsive member, StvacINV1, regulates cold-induced sweetening of tubers
Systematic analysis of potato acid invertase genes reveals that a cold-responsive member, StvacINV1, regulates cold-induced sweetening of tubers
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DOI：
10.1007/s00438-011-0632-1
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发表时间：
2011-08-01
影响因子：
3.1
通讯作者：
Li, Xiu-Qing
中科院分区：
文献类型：
--
作者：
Liu, Xun;Zhang, Chi;Li, Xiu-Qing
酸转化酶被认为在植物发育过程中起调节作用，并对环境刺激作出反应。目前还没有完整的酸性转化酶家族在马铃薯中的表达谱。通过检索现有数据库，确定马铃薯中至少有6个酸性转化酶基因，包括4个细胞壁转化酶基因和2个液泡转化酶基因。为了挖掘它们的潜在功能，我们对它们在马铃薯植株各器官和贮藏块茎中的表达谱进行了比较分析。结果表明，每个基因都表现出独特的表达模式，这些表达模式在转录丰度或器官特异性方面存在差异，表明单个基因可能参与了植物发育。液泡转化酶基因StvacINV1在马铃薯块茎中表达量最高。进一步的贮藏实验表明，低温对StvacINV1有强烈的诱导作用，这与低温贮藏块茎中葡萄糖的积累一致。马铃薯反义转化对StvacINV1的抑制证实了转基因块茎中StvacINV1转录物丰度较低与还原糖含量较低和切块颜色较浅有关。这些证据有力地表明StvacINV1是一个参与冷诱导马铃薯块茎变甜调控的基因。这为研究冷致甜性状的调控机制和农艺改良提供了途径。
Acid invertase is believed to play a regulatory role during plant developmental processes and to respond to environmental stimuli. The expression profiles of the entire acid invertase family are not yet available for potato. By searching existing databases, it was determined that there are at least six acid invertase genes in potato, including four cell-wall invertase genes and two vacuolar invertase genes. They were subjected to comparative expression profiling in various organs of potato plants and in stored tubers to exploit their potential functions. The results revealed that each gene exhibited a unique expression pattern, which differed in transcript abundance or showed organ-specific features, pointing to the possible involvement of individual genes in plant development. The vacuolar invertase gene StvacINV1 had the highest expression level among three genes detected in the potato tubers. Further storage experiments showed that StvacINV1 was strongly induced by low temperatures, which is consistent with glucose accumulation in cold-stored tubers. Suppression of StvacINV1 by the antisense transformation in potato confirmed that lower StvacINV1 transcript abundance in transgenic tubers is related to lower reducing sugar content and lighter chip color in comparison with the wild type. The evidence strongly suggests that StvacINV1 is a gene involved in regulation of cold-induced sweetening of potato tubers. This provides an avenue for studying the mechanism involved in the regulation of the cold-induced sweetening trait and for agronomic enhancement.