Mismatch repair protein expression and colorectal cancer in Hispanics from Puerto Rico.
Mismatch repair protein expression and colorectal cancer in Hispanics from Puerto Rico.
复制标题
波多黎各西班牙裔人的错配修复蛋白表达与结直肠癌。
DOI：
10.1007/s10689-009-9310-4
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发表时间：
2010-06
期刊：
影响因子：
2.2
通讯作者：
Cruz-Correa, Marcia
中科院分区：
文献类型：
--
作者：
De Jesus-Monge, Wilfredo E.;Gonzalez-Keelan, Carmen;Zhao, Ronghua;Hamilton, Stanley R.;Rodriguez-Bigas, Miguel;Cruz-Correa, Marcia
结直肠癌（CRC）是发病率和死亡率的主要原因，错配修复（MMR）基因的改变导致蛋白缺失（阴性）表达，约占结直肠癌病例的20%。免疫组织化学是一种用于CRC中MMR蛋白表达预筛选的工具，但关于其在西班牙裔中的应用的文献很少。然而，西班牙裔是美国的第二大种族，CRC是该群体的公共卫生负担。我们的目的是确定MMR蛋白阴性CRC的频率，并评估其与波多黎各西班牙裔患者临床和病理特征的关系，这是我们所知的第一次。对波多黎各医疗中心2001年至2005年未选择的结直肠癌患者进行了回顾性观察研究。MLH1和MSH2是最常见的MMR基因，在没有MLH1蛋白表达的情况下，使用免疫组织化学、微卫星不稳定性（MSI）和BRAF基因分析来评估蛋白表达。对164例结直肠癌患者进行评估：总体MMR蛋白阴性频率为4.3%，mlh1蛋白阴性表达、msi -高、BRAF基因正常同时出现的频率为0.6%。MMR蛋白阴性表达与结肠近端位置（p = 0.02）和肿瘤组织学分化差（p = 0.001）相关，但与其他特征无关。波多黎各西班牙裔人群中MMR蛋白阴性结直肠癌的发生率低于其他人群。这一发现可以解释为什么美国西班牙裔的CRC发病率低于非西班牙裔的白人和黑人。
Colorectal cancer (CRC) is a leading cause of morbidity and mortality and alterations in mismatch repair (MMR) genes, leading to absent protein (negative) expression, are responsible for approximately 20% of CRC cases. Immunohistochemistry is a tool for prescreening of MMR protein expression in CRC but the literature on its use on Hispanics is scarce. However, Hispanics represent the second leading ethnicity in the United States (US) and CRC is a public health burden in this group. Our objectives were to determine the frequency of MMR protein-negative CRC and to evaluate its association with clinical and pathological characteristics among Hispanics from Puerto Rico, for the first time to our knowledge. A retrospective observational study of unselected CRC patients from the Puerto Rico Medical Center from 2001 to 2005 was done. MLH1 and MSH2, the most commonly altered MMR genes, protein expression was evaluated using immunohistochemistry, with microsatellite instability (MSI) and BRAF gene analyses in the absence of MLH1 protein expression. One-hundred sixty-four CRC patients were evaluated: the overall MMR protein-negative frequency was 4.3%, with 0.6% frequency of co-occurrence of MLH1-protein negative expression, MSI-high, and normal BRAF gene. MMR protein-negative expression was associated with proximal colon location (p = 0.02) and poor histological tumor differentiation (p = 0.001), but not with other characteristics. The frequency of MMR protein-negative CRC in Hispanics from Puerto Rico was lower than reported in other populations. This finding may explain the lower CRC incidence rate among US Hispanics as compared to US non-Hispanic whites and blacks.
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影响因子：
29.4
作者：
Bermejo, JL;Eng, C;Hemminki, K
通讯作者：
Hemminki, K
影响因子：
6.2
作者：
Christensen, M;Katballe, N;Orntoft, TF
通讯作者：
Orntoft, TF
影响因子：
37.3
作者：
Brim H;Mokarram P;Naghibalhossaini F;Saberi-Firoozi M;Al-Mandhari M;Al-Mawaly K;Al-Mjeni R;Al-Sayegh A;Raeburn S;Lee E;Giardiello F;Smoot DT;Vilkin A;Boland CR;Goel A;Hafezi M;Nouraie M;Ashktorab H
通讯作者：
Ashktorab H
影响因子：
6.4
作者：
Ashktorab, H;Smoot, DT;Giardiello, FM
通讯作者：
Giardiello, FM
影响因子：
3.1
作者：
Ashktorab, Hassan;Brim, Hassan;Raeburn, Sandy
通讯作者：
Raeburn, Sandy