Shoot control of root development and nodulation is mediated by a receptor-like kinase
Shoot control of root development and nodulation is mediated by a receptor-like kinase
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DOI：
10.1038/nature01207
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发表时间：
2002-11-28
期刊：
影响因子：
64.8
通讯作者：
Stougaard, J
中科院分区：
文献类型：
--
作者：
Krusell, L;Madsen, LH;Stougaard, J
在豆科植物中，根瘤器官发生是由细菌（俗称根瘤菌）合成的脂几丁质低聚糖（lipochitin oligosaccharides）激活的(1)。成功的共生相互作用导致形成高度特化的器官，称为根瘤，它为共生固氮提供了独特的环境。在野生型植物中，根瘤的数量受一种信号机制的调节，该机制整合了环境和发育线索，以阻止根的易感区域内的大多数根瘤菌感染（2-7）。此外，一个反馈机制控制着根系对侵染的时空易感性。这种机制被称为结瘤的自动调节，因为早期的结瘤事件抑制了较年轻的根组织的结瘤（3,4,8）。在多结异常根（har1）位点发生突变的日本莲子植株逃脱了这种调节，形成了过多的结节（9-11）。本文报道了HAR1基因与豌豆同源基因Pisum sativum， SYM29的克隆及表达分析。HAR1编码一种丝氨酸/苏氨酸受体激酶，该激酶是根生长、根瘤数量和共生发育的硝酸盐敏感性的调控所必需的。
In legumes, root nodule organogenesis is activated in response to morphogenic lipochitin oligosaccharides that are synthesized by bacteria, commonly known as rhizobia(1). Successful symbiotic interaction results in the formation of highly specialized organs called root nodules, which provide a unique environment for symbiotic nitrogen fixation. In wild-type plants the number of nodules is regulated by a signalling mechanism integrating environmental and developmental cues to arrest most rhizobial infections within the susceptible zone of the root(2-7). Furthermore, a feedback mechanism controls the temporal and spatial susceptibility to infection of the root system. This mechanism is referred to as autoregulation of nodulation, as earlier nodulation events inhibit nodulation of younger root tissues(3,4,8). Lotus japonicus plants homozygous for a mutation in the hypernodulation aberrant root (har1) locus escape this regulation and form an excessive number of nodules(9-11). Here we report the molecular cloning and expression analysis of the HAR1 gene and the pea orthologue, Pisum sativum, SYM29. HAR1 encodes a putative serine/threonine receptor kinase, which is required for shoot-controlled regulation of root growth, nodule number, and for nitrate sensitivity of symbiotic development.