Importation of taxadiene synthase into chloroplast improves taxadiene production in tobacco
Importation of taxadiene synthase into chloroplast improves taxadiene production in tobacco
复制标题
将紫杉二烯合酶导入叶绿体可提高烟草中紫杉二烯的产量
DOI：
10.1007/s00425-021-03626-z
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发表时间：
2021-05-01
期刊：
影响因子：
4.3
通讯作者：
Chang, Ling
中科院分区：
文献类型：
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作者：
Fu, Jinqiu;Xu, Wenbo;Chang, Ling
主要结论将紫杉二烯合酶导入叶绿体对于紫杉二烯的高效异源生产具有重要意义。 摘要紫杉二烯是紫杉醇的第一个前体，是由香叶基香叶基焦磷酸（GGPP）在紫杉二烯合酶（TS）的作用下合成的。紫杉二烯的异源生产可能依赖于胞质甲羟戊酸（MVA）途径和质体甲基赤藓糖醇磷酸（MEP）途径。我们建议叶绿体中的分区工程作为紫杉二烯生产的有效方法。在本研究中，我们将短叶红豆杉的TS基因直接导入烟草叶绿体基因组中，发现转质体植物积累了较低含量的紫杉二烯，为 ~ 5.6 μg/g干重（DW）。此外，我们尝试了 MEP 和 MVA 途径的组合，通过将截短版本的 TS（不编码转运肽）核转化到转质体植物中来合成紫杉二烯。然而，这并没有进一步提高紫杉二烯的产量。相比之下，我们发现表达带有叶绿体转运肽的TS的转基因植物的叶子中紫杉二烯的产量高达87.8 μg/g DW，明显高于转质体植物的叶子。因此，本研究强调了将 TS 输入叶绿体以生产紫杉二烯的重要性。
Main conclusionImportation of taxadiene synthase into chloroplasts is important for the efficient heterologous production of taxadiene.AbstractTaxadiene, the first committed precursor to taxol, is synthesized from geranylgeranyl pyrophosphate (GGPP) by action of taxadiene synthase (TS). Heterologous production of taxadiene could potentially rely on both cytosolic mevalonic acid (MVA) pathway and the plastidic methylerythritol phosphate (MEP) pathway. We suggest the compartmentalized engineering in chloroplast as an efficient approach for taxadiene production. In this study, we directly introduced theTSgene fromTaxus brevifoliainto the tobacco chloroplast genome and found that the transplastomic plants accumulated a low content of taxadiene, ~ 5.6 μg/g dry weight (DW). Moreover, we tried a combination of MEP and MVA pathways for taxadiene synthesis by nuclear transformation with a truncated version ofTS(without encoding a transit peptide) into the transplastomic plants. However, this did not further improve the taxadiene production. In contrast, we found that taxadiene could be produced up to 87.8 μg/g DW in leaves of transgenic plants expressingTSwith a chloroplast transit peptide, which was significantly higher than that in leaves of transplastomic plants. Thus, this study highlights the importance of TS importation into chloroplast for production of taxadiene.