Quantitation of Mycotoxins in Food and Feed from Burkina Faso and Mozambique Using a Modern LC-MS/MS Multitoxin Method
Quantitation of Mycotoxins in Food and Feed from Burkina Faso and Mozambique Using a Modern LC-MS/MS Multitoxin Method
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DOI：
10.1021/jf302003n
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发表时间：
2012-09-12
影响因子：
6.1
通讯作者：
Krska, Rudolf
中科院分区：
文献类型：
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作者：
Warth, Benedikt;Parich, Alexandra;Krska, Rudolf
在这项研究中，LC-MS/MS多毒素方法涵盖了247真菌和细菌的代谢产物，应用于分析不同的食品和饲料从布基纳法索和莫桑比克。总的来说，在122个主要是玉米和花生的样品和一些高粱、小米、大米、小麦、大豆、干果、其他加工食品和动物饲料的样品中测定了63种代谢物。在玉米中观察到的黄曲霉毒素B-1（布基纳法索，50%发生率，中位数= 23.6 μ g/kg;莫桑比克，46%发生率，中位数= 69.9 μ g/kg）比在花生中观察到的黄曲霉毒素（布基纳法索，22%发生率，中位数= 10.5 μ g/kg;莫桑比克，14%发生率，中位数= 3.4 μ g/kg）更频繁。莫桑比克玉米中伏马菌素B-1浓度（发病率92%，中位数= 869 μ g/kg）高于布基纳法索（发病率81%，中位数= 269 μ g/kg）。此外，还检测到赭曲霉毒素A、玉米赤霉烯酮、脱氧雪腐镰刀菌烯醇、雪腐镰刀菌烯醇和其他较少报道的真菌毒素，如桔霉素、交链孢霉醇、环匹阿尼酸、杂色曲霉素、念珠藻素、白僵菌素和恩镰孢菌素。多达28个有毒真菌代谢产物定量在一个单一的样品，强调各种各样的真菌毒素共曝光。大多数真菌毒素在这两个国家以前都没有报道过。
In this study an LC-MS/MS multitoxin method covering a total of 247 fungal and bacterial metabolites was applied to the analysis of different foods and feedstuffs from Burkina Faso and Mozambique. Overall, 63 metabolites were determined in 122 samples of mainly maize and groundnuts and a few samples of sorghum, millet, rice, wheat, soy, dried fruits, other processed foods and animal feeds. Aflatoxin B-1 was observed more frequently in maize (Burkina Faso, 50% incidence, median = 23.6 mu g/kg; Mozambique, 46% incidence, median = 69.9 mu g/kg) than in groundnuts (Burkina Faso, 22% incidence, median = 10.5 mu g/kg; Mozambique, 14% incidence, median = 3.4 mu g/kg). Fumonisin B-1 concentrations in maize were higher in Mozambique (92% incidence, median = 869 mu g/kg) than in Burkina Faso (81% incidence, median = 269 mu g/kg). In addition, ochratoxin A, zearalenone, deoxynivalenol, nivalenol, and other less reported mycotoxins such as citrinin, alternariol, cyclopiazonic acid, sterigmatocystin, moniliformin, beauvericin, and enniatins were detected. Up to 28 toxic fungal metabolites were quantitated in a single sample, emphasizing the great variety of mycotoxin coexposure. Most mycotoxins have not been reported before in either country.