The potential for manipulating RNA with pentatricopeptide repeat proteins
The potential for manipulating RNA with pentatricopeptide repeat proteins
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DOI：
10.1111/tpj.12377
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发表时间：
2014-06-01
期刊：
影响因子：
7.2
通讯作者：
Small, Ian
中科院分区：
文献类型：
--
作者：
Yagi, Yusuke;Nakamura, Takahiro;Small, Ian
五肽重复 (PPR) 蛋白家族在植物中特别普遍，包括许多参与细胞器 RNA 代谢各个方面的序列特异性 RNA 结合蛋白，包括 RNA 稳定性、加工、编辑和翻译。 PPR 蛋白由 2-30 个 PPR 基序串联组成，每个基序与 RNA 靶标中的一个核苷酸对齐。每个基序中两个或三个特定位置的氨基酸侧链以可预测和可编程的方式赋予核苷酸特异性。因此，PPR 蛋白似乎为创建具有定制特异性的定制 RNA 结合蛋白提供了极有前途的机会。我们总结了 PPR 蛋白对 RNA 识别的最新进展，特别关注基于 PPR 的 RNA 操作工具的潜在应用，以及科学界常规使用这些工具之前仍需克服的挑战。
The pentatricopeptide repeat (PPR) protein family, which is particularly prevalent in plants, includes many sequence-specific RNA-binding proteins involved in all aspects of organelle RNA metabolism, including RNA stability, processing, editing and translation. PPR proteins consist of a tandem array of 2-30 PPR motifs, each of which aligns to one nucleotide in the RNA target. The amino acid side chains at two or three specific positions in each motif confer nucleotide specificity in a predictable and programmable manner. Thus, PPR proteins appear to provide an extremely promising opportunity to create custom RNA-binding proteins with tailored specificity. We summarize recent progress in understanding RNA recognition by PPR proteins, with a particular focus on potential applications of PPR-based tools for manipulating RNA, and on the challenges that remain to be overcome before these tools may be routinely used by the scientific community.