Selective conservation of an E-protein gene promoter during vertebrate evolution.
Selective conservation of an E-protein gene promoter during vertebrate evolution.
复制标题
脊椎动物进化过程中 E 蛋白基因启动子的选择性保守。
DOI：
10.1016/s0014-5793(98)01417-3
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发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
Neuman,T
中科院分区：
文献类型：
--
作者：
Shain,DH;Zuber,MX;Norris,J;Yoo,J;Neuman,T
小鼠e蛋白基因ME1编码与形态发育相关的非组织特异性螺旋-环-螺旋转录因子。ME1基因的表达受一个TATA-less启动子调控，该启动子包含多个Sp1共识元件、e -box和一个新的转录起始位点。在这项研究中，我们比较了从蛙到人的脊椎动物ME1启动子的同源DNA。在ME1转录起始位点（ME1 Inr）对应的区域中发现了一个惊人的序列相似性区域。在该区域内，一个poly d(a)通道和一个9-bp的反向重复序列（5 ' -GTCCGCCTG）在所有被检测的物种中高度保守。识别这些DNA元素的蛋白复合物存在于遥远的脊椎动物（青蛙、小鸡、猴子和人类）中，并且能够将ME1 Inr弯曲到与先前描述的小鼠MBPα和MBPβ蛋白相似的程度（~ 60°）。总的来说，这些结果表明，ME1 inr样元件及其相关蛋白在3.5亿多年前的祖先脊椎动物中起作用。
The murine E-protein gene ME1 encodes a non-tissue-specific, helix-loop-helix transcription factor that is associated with morphological development. ME1 gene expression is regulated by a TATA-less promoter that contains multiple Sp1 consensus elements, E-boxes, and a novel transcription initiation site. In this study, we compared DNA homologous to the ME1 promoter from vertebrate species ranging from frog to human. A region of striking sequence similarity was identified in a region corresponding to the ME1 transcription initiation site (ME1 Inr). Within this region, a poly d(A) tract and a 9-bp inverted repeat (5′-GTCCGCCTG) were highly conserved in all species that were examined. Protein complexes that recognized these DNA elements were present among distant vertebrates (frog, chick, monkey and human), and were able to bend the ME1 Inr to a similar extent (∼60°) as the previously described murine MBPα and MBPβ proteins. Collectively, these results suggest that an ME1 Inr-like element and its associated proteins functioned in an ancestral vertebrate more than 350 million years ago.