Point mutation generates constitutive expression of an inducible eukaryotic gene.
Point mutation generates constitutive expression of an inducible eukaryotic gene.
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点突变产生诱导型真核基因的组成型表达。
DOI：
10.1073/pnas.82.3.643
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发表时间：
1985
影响因子：
11.1
通讯作者：
Cooper,TG
中科院分区：
文献类型：
--
作者：
Sumrada,RA;Cooper,TG
我们描述了对两种顺式显性突变的分析，这些突变导致酵母中诱导型 CAR1 基因的组成型表达。一种突变是由于在 CAR1 特异性转录开始点的上游插入一个 Ty 元件而产生的。另一个突变是 -153 位的 C 到 G 颠换。在 CAR1 转录区域之外分离到该点突变表明该基因的表达在转录时受到调节。它还证明了在逐个核苷酸的基础上分析真核基因调控序列的可行性和有用性。
We describe the analysis of two cis-dominant mutations that result in constitutive expression of the inducible CAR1 gene from yeast. One mutation results from insertion of a Ty element just upstream from the point where CAR1-specific transcription begins. The other mutation is a C-to-G transversion at position -153. Isolation of this point mutation, outside of the transcribed region of CAR1, suggests that expression of this gene is regulated at transcription. It also demonstrates the feasibility and usefulness of analyzing the regulatory sequences of eukaryotic genes on a nucleotide-by-nucleotide basis.