Spinal sensory neurons express multiple sodium channel alpha-subunit mRNAs
Spinal sensory neurons express multiple sodium channel alpha-subunit mRNAs
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DOI：
10.1016/s0169-328x(96)00163-5
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发表时间：
1996-12-31
期刊：
影响因子：
--
通讯作者：
Waxman, SG
中科院分区：
文献类型：
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作者：
Black, JA;DibHajj, S;Waxman, SG
采用原位杂交和逆转录聚合酶链反应（RT-PCR）技术检测了1 d离体培养成年大鼠DRG神经元中钠通道α -、β 1-和β 2亚基mrna的表达。结果表明，钠通道α亚基mrna在体外培养1天的小（45 μ m直径）DRG神经元中存在差异表达（div）。钠通道mRNA I在大神经元中的表达水平高于小DRG神经元，而钠通道mRNA II的表达是可变的，大多数细胞缺乏或表现出这些mRNA的低水平可检测信号，并且有限数量的神经元具有中等表达水平。DRG神经元对钠通道mRNA m的杂交信号通常可忽略或低水平。钠通道mRNA Na6和NaG的表达模式相似，大多数大DRG神经元和许多中等DRG神经元表现出高水平的表达。人钠通道大鼠同源基因hNE-Na mRNA几乎在每个DRG神经元中都检测到；所有大小类别的大多数细胞都表现出中等或高水平的hNE-Na表达。钠通道SNS mRNA在所有大小类别的DRG神经元中均有表达，但在小、中DRG神经元中的表达高于在大神经元中的表达。在大多数DRG神经元中，无论大小，均未检测到与小鼠钠通道mRNA同源的大鼠mRNA 2.3，或低水平检测到，尽管在一些神经元中检测到适度表达。钠通道β 1-和β 2亚基mrna表现出相似的表达模式；它们在大多数DRG神经元中检测到，尽管大神经元的表达水平往往高于小神经元。完整成人DRG的RT-PCR和原位杂交显示，钠通道mrna的表达模式与体外DRG神经元相似。这些结果表明，成人DRG神经元在体外和原位表达多种钠通道mrna，并提示在这些细胞中观察到的钠电流的生物物理异质性的分子基础。
The expression of sodium channel alpha-, beta 1- and beta 2-subunit mRNAs was examined in adult rat DRG neurons in dissociated culture at I day in vitro and within sections of intact ganglia by in situ hybridization and reverse transcription polymerase chain reaction (RT-PCR). The results demonstrate that sodium channel alpha-subunit mRNAs are differentially expressed in small (45 mu m diam.) cultured DRG neurons at 1 day in vitro (div). Sodium channel mRNA I is expressed at higher levels in large neurons than small DRG neurons, while sodium channel mRNA II is variably expressed, with most cells lacking or exhibiting low levels of detectable signal of these mRNAs and limited numbers of neurons with moderate expression levels. DRG neurons generally exhibit negligible or low levels of hybridization signal for sodium channel mRNA m. Sodium channel mRNAs Na6 and NaG show similar patterns of expression, with most large and many medium DRG neurons exhibiting high levels of expression. The mRNA for the rat cognate of human sodium channel hNE-Na is detected in virtually every DRG neuron; most cells in all size classes exhibit moderate or high levels of hNE-Na expression. Sodium channel SNS mRNA is expressed in all size classes of DRG neurons, but shows greater expression in small and medium DRG neurons than in large neurons. The mRNA for the rat cognate of mouse sodium channel mNa,2.3 is not detected, or is detected at low levels, in most DRG neurons, regardless of size, although moderate expression is detected in some neurons. Sodium channel beta 1- and beta 2-subunit mRNAs exhibit similar expression patterns; they are detected in most DRG neurons, although the level of expression tends to be greater in large neurons than in small neurons. RT-PCR and in situ hybridization of intact adult DRG showed a similar pattern of expression of sodium channel mRNAs to that observed in DRG neurons in vitro. These results demonstrate that adult DRG neurons express multiple sodium channel mRNAs in vitro and in situ and suggest a molecular basis for the biophysical heterogeneity of sodium currents observed in these cells.