Kinesin's biased stepping mechanism: Amplification of neck linker zippering
Kinesin's biased stepping mechanism: Amplification of neck linker zippering
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DOI：
10.1529/biophysj.106.087049
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发表时间：
2006-10-01
影响因子：
3.4
通讯作者：
Fox, Ronald F.
中科院分区：
文献类型：
--
作者：
Mather, William H.;Fox, Ronald F.
在修正布朗运动的框架内开发了驱动蛋白运动功能的物理驱动模型。该模型解释了颈部连接器拉链的放大如何通过众所周知的过阻尼动力学公式自然产生，从而提供了一种方法来了解弱有利拉链如何导致游离驱动蛋白头与微管的强烈正向结合。驱动蛋白运动的其他方面，例如头部协调和限速步骤，与 ATP 与微管结合头部结合的力依赖性抑制直接相关。修正布朗运动模型作为动力冲程模型的替代模型提出，并为驱动蛋白步进循环中 ATP 水解的重要性提供了另一种解释。
A physically motivated model of kinesin's motor function is developed within the framework of rectified Brownian motion. The model explains how the amplification of neck linker zippering arises naturally through well-known formulae for overdamped dynamics, thereby providing a means to understand how weakly-favorable zippering leads to strongly favorable plus-directed binding of a free kinesin head to microtubule. Additional aspects of kinesin's motion, such as head coordination and rate-limiting steps, are directly related to the force-dependent inhibition of ATP binding to a microtubule bound head. The model of rectified Brownian motion is presented as an alternative to power stroke models and provides an alternative interpretation for the significance of ATP hydrolysis in the kinesin stepping cycle.