Regional localization of 188 sequence tagged sites on a somatic cell hybrid mapping panel for human chromosome 3.
Regional localization of 188 sequence tagged sites on a somatic cell hybrid mapping panel for human chromosome 3.
复制标题
人类 3 号染色体体细胞杂交作图面板上 188 个序列标记位点的区域定位。
DOI：
10.1006/geno.1994.1665
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发表时间：
1994
期刊：
影响因子：
4.4
通讯作者：
Lewis,TB
中科院分区：
文献类型：
--
作者：
Leach,RJ;Chinn,R;Reus,BE;Hayes,S;Schantz,L;Dubois,B;Overhauser,J;Ballabio,A;Drabkin,H;Lewis,TB
3号染色体占基因组的7%，包含至少210 Mb的DNA。为了加快对这条染色体的分析，我们组装了一个体细胞杂交图谱，用总共19个杂交体将人类3号染色体细分为23个区间。从16个包含3号染色体易位的患者细胞中构建杂交种。所有这些杂交种都选择性地保留了衍生染色体。此外，我们还利用了2个辐射还原杂交种和3个携带人类3号染色体与啮齿动物染色体之间自发易位的杂交种。整个面板有9个短臂断点，涉及频带p24.2、p22、p21、p14和p12，还有11个长臂断点，涉及频带q13、q21、q25、q26和q27。此外，两个细胞系似乎在着丝粒或着丝粒附近有断点。迄今为止，我们已经使用该面板在短臂上定位了92个序列，在长臂上定位了89个序列，在着丝粒附近定位了7个序列。这些杂交体是一种有用的工具，可以快速定位3号染色体上的标记，并极大地协助其他制图工作。
Chromosome 3 comprises 7% of the genome and contains at least 210 Mb of DNA. To expedite the analysis of this chromosome, we have assembled a somatic cell hybrid mapping panel that subdivides human chromosome 3 into 23 intervals using a total of 19 hybrids. Hybrids were constructed from 16 patients' cells containing chromosome 3 translocations. All of these hybrids selectively retained the derivative 3 chromosome. In addition, we utilized 2 radiation-reduced hybrids and 3 hybrids carrying spontaneous translocations between human chromosome 3 and rodent chromosomes. The entire panel has 9 short arm breakpoints that involve bands p24.2, p22, p21, p14, and p12 plus a total of 11 long arm breakpoints that involve bands q13, q21, q25, q26, and q27. In addition, two cell lines appear to have breakpoints at or near the centromere. To date, we have used this panel to localize 92 sequences regionally on the short arm, 89 sequences on the long arm, and 7 sequences near the centromere. These hybrids are useful tools that allow the rapid localization of markers on chromosome 3 and greatly assist other mapping efforts.