17 beta-estradiol protects nucleus pulposus cells from serum deprivation-induced apoptosis and regulates expression of MMP-3 and MMP-13 through promotion of autophagy
17 beta-estradiol protects nucleus pulposus cells from serum deprivation-induced apoptosis and regulates expression of MMP-3 and MMP-13 through promotion of autophagy
复制标题
17β-雌二醇保护髓核细胞免受血清剥夺诱导的细胞凋亡，并通过促进自噬调节 MMP-3 和 MMP-13 的表达
DOI：
10.1016/j.bbrc.2018.06.077
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发表时间：
2018-09-05
影响因子：
3.1
通讯作者：
Cheng, Xigao
中科院分区：
文献类型：
--
作者：
Ao, Peng;Huang, Wenzhou;Cheng, Xigao
血清剥夺可能是椎间盘退变（IVD）的原因之一。17 β-雌二醇（E2）已被注意到可保护髓核细胞（NPC）免受凋亡。自噬和凋亡在维持IVD的稳态中起着至关重要的作用。到目前为止，关于自噬是否在E2介导的NPC保护中发挥作用的研究很少。本研究的目的是了解自噬是否参与E2对血清剥夺诱导的细胞凋亡和基质金属蛋白酶（MMP）-3和MMP-13表达的保护作用。mCherry-GFP-LC 3-腺病毒转染用于监测自噬检测。Western blotting检测细胞自噬相关蛋白的表达水平，流式细胞术和Western blotting检测细胞凋亡和MMPs的表达。相应地，在血清剥夺条件下，NP细胞出现了自噬和凋亡，自噬发生率在48 h时开始达到峰值，凋亡和MMPs发生率在E2（10（-7）M）处理后开始达到最低值。而E2和3-MA的联合使用导致自噬显著减少，而凋亡和MMPs的表达水平异常升高。提示血清剥夺可诱导大鼠NPC细胞凋亡和MMP-3、MMP-13的表达，E2可通过促进自噬而有效抑制MMP-3、MMP-13的表达。(C)2018爱思唯尔公司All rights reserved.
Serum deprivation is a likely contributor to intervertebral disc (IVD) degeneration (IVDD).17 beta-estradiol (E2) have been noted to protect nucleus pulposus cells (NPCs) against apoptosis. Autophagy and apoptosis play a paramount role in maintaining the homeostasis of IVD. So far, little research has been published on whether autophagy plays a role for the E2 mediated protection of NPCs. The aim of this study is to understand whether autophagy is involved in the protective effect of E2 against serum deprivation-induced cell apoptosis and expression of matrix metalloproteinase (MMP)-3 and MMP-13. mCherry-GFP-LC3-adenovirus transfection is used to monitor autophagy detection. The expression levels of autophagy-related proteins were measured by Western blotting, Apoptosis and MMPs were detected by flow cytometry and Western blotting. Accordingly, Autophagy and apoptosis was detected in NP cells under serum deprivation conditions, the autophagy incidence began to reached a peak value at 48 h, the apoptosis and MMPs incidence began reached a minimum value treat with E2 (10(-7) M). Whereas the combined use of E2 and 3-MA led to a dramatic decrease in autophagy, while aberrantly elevated expression levels of apoptotic and MMPs. These data suggest that serum deprivation-induced apoptosis and MMP-3, MMP-13, which was efficiently suppressed by the E2 through promoting autophagy in rat NPCs. (C) 2018 Elsevier Inc. All rights reserved.