Cancer stem-like cells contribute to cisplatin resistance and progression in bladder cancer
Cancer stem-like cells contribute to cisplatin resistance and progression in bladder cancer
复制标题
癌症干细胞样细胞有助于膀胱癌的顺铂耐药性和进展
DOI：
10.1016/j.canlet.2012.02.010
复制
发表时间：
2012-09-01
期刊：
影响因子：
9.7
通讯作者：
Yang, Jin
中科院分区：
文献类型：
--
作者：
Zhang, Yi;Wang, Zhi;Yang, Jin
多种癌症干细胞样细胞（CSC）已被证明与癌症的致瘤性、复发和转移有关。尽管有几份报告证明人类膀胱癌中存在 CSC，但它们的身份仍然存在争议，而且很少有研究探讨它们在顺铂耐药和相关肿瘤进展中的作用。在这项研究中，从膀胱细胞系 T24 中选择顺铂后，CSC 亚群得到富集。顺铂耐药的 T24 细胞表现出更强的自我更新能力，如更高水平的球体形成和干细胞标记物表达所证明，包含更大比例的侧群细胞并表现出更高的致瘤性。它们还具有上皮-间质转化特征。此外，Bmi1和Nanog的表达水平与尿路上皮细胞癌组织的恶性程度之间存在很强的相关性。我们提供了第一个直接证据，证明 CSC 样细胞存在于顺铂耐药的膀胱癌细胞群中，并且可能在膀胱癌的进展和耐药性中发挥作用。 (C) 2012 Elsevier Ireland Ltd. 保留所有权利。
A variety of cancer stem-like cells (CSCs) have been shown to be responsible for cancer tumorigenicity, relapse and metastasis. Despite several reports demonstrating the presence of CSCs in human bladder cancer, their identities are still under debate, and few studies have examined their roles in cisplatin resistance and related tumor progression. In this study, a subpopulation of CSCs was enriched following cisplatin selection from the bladder cell line T24. The cisplatin-resistant T24 cells displayed a greater self-renewal capacity as demonstrated by higher levels of sphere formation and stem cell marker expression, contained a larger proportion of side population cells and exhibited higher tumorigenicity. They also possessed epithelial-mesenchymal transition characteristics. Furthermore, a strong correlation between the levels of Bmi1 and Nanog expression and the degree of malignancy of urothelial cell carcinomas tissues was observed. We provide the first direct evidence that CSC-like cells exist in the population of cisplatin-resistant bladder cancer cells and may play a role in the progression and drug resistance of bladder cancer. (C) 2012 Elsevier Ireland Ltd. All rights reserved.