IMMUNOHISTOCHEMICAL EVIDENCE FOR SEPARATE POPULATIONS OF SOMATOSTATIN-CONTAINING AND SUBSTANCE P-CONTAINING PRIMARY AFFERENT NEURONS IN RAT
IMMUNOHISTOCHEMICAL EVIDENCE FOR SEPARATE POPULATIONS OF SOMATOSTATIN-CONTAINING AND SUBSTANCE P-CONTAINING PRIMARY AFFERENT NEURONS IN RAT
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DOI：
10.1016/0306-4522(76)90008-7
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发表时间：
1976-01-01
期刊：
影响因子：
3.3
通讯作者：
ARIMURA, A
中科院分区：
文献类型：
--
作者：
HOKFELT, T;ELDE, R;ARIMURA, A
用间接免疫荧光技术研究了生长抑素和P物质两种小肽的定位。这两种肽存在于脊神经节中的小神经元细胞体中、脊髓背角中的纤维中和肠壁中的纤维中。通过比较连续切片分别与生长抑素和P物质的抗血清孵育，确定生长抑素或生长抑素样免疫反应性和P物质或P物质样免疫反应性存在于不同的细胞中。这也可能由背角中免疫反应阳性纤维的某种差异性分布表明：在板层II中观察到最高浓度的生长抑素阳性纤维，而在板层I中也存在丰富的P物质阳性纤维。此外，许多物质P-，但没有生长抑素阳性纤维，被发现周围的中央管和腹角。在肠壁中，P物质阳性纤维多于生长抑素阳性纤维，提示初级感觉神经元存在两个亚群，一个含有生长抑素或生长抑素样免疫反应性，另一个含有P物质或P物质样免疫反应性。
The localization of two small peptides, somatostatin and substance P, has been studied with the indirect immunofluorescence technique. Both peptides were present in small neuronal cell bodies in spinal ganglia, in fibers in the dorsal horn of the spinal cord and in fibers in the intestinal wall. By comparing consecutive sections incubated with antisera to somastostatin and to substance P respectively, it was established that somatostatin, or somatostatin-like immunoreactivity and substance P, or substance P-like immunoreactivity are present in different cells. This is possibly indicated also by a somewhat differential distribution of the immunoreactive fibers in the dorsal horn: the highest concentration of somatostatin-positive fibers was observed in lamina II, whereas abundant substance P-positive fibers were present also in lamina I. Furthermore, numerous substance P-, but no somatostatin-positive fibers, were found around the central canal and in the ventral horns. In the intestinal wall more substance P-positive than somatostatin-positive fibers were seen.The present results indicate that two subpopulations of primary sensory neurons exist, one containing somatostatin, or somatostatin-like immunoreactivity, and the other containing substance P, or substance P-like immunoreactivity.