Effects of tank color on melanin-concentrating hormone levels in the brain, pituitary gland, and plasma of the barfin flounder as revealed by a newly developed time-resolved fluoroimmunoassay
Effects of tank color on melanin-concentrating hormone levels in the brain, pituitary gland, and plasma of the barfin flounder as revealed by a newly developed time-resolved fluoroimmunoassay
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DOI：
10.1016/j.ygcen.2005.04.012
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发表时间：
2005-09-15
影响因子：
2.7
通讯作者：
Yamamori, K
中科院分区：
文献类型：
--
作者：
Amiya, N;Amano, M;Yamamori, K
在白色池塘饲养的巴鳍比目鱼Verasper Moseri具有较小的非眼侧皮肤色素面积比例，生长速度较快，脑内黑色素浓缩激素(MCH)免疫反应细胞体和MCH基因表达较多，表明白色池塘养殖的鱼MCH的合成和释放较高。本研究建立了MCH的时间分辨荧光免疫分析方法。对在白色或黑色水箱中饲养的巴鳍鲨的大脑、脑下垂体和血浆中的MCH水平进行了评估。使用两种抗体在固相二抗、MCH抗体、样品和Eu标记的MCH之间进行竞争分析。巴氏比目鱼连续稀释的提取物(脑、脑垂体腺和血浆)的位移曲线与MCH标准的位移曲线平行。白箱饲养5个月的鱼脑和血浆中的MCH水平均高于黑箱。这些结果表明，在白色背景下，MCH的合成和分泌增加，并且MCH既参与了身体的生长，也参与了皮肤的色素沉积。(C)2005 Elsevier Inc.保留所有权利。
A pleuronectiform fish, the barfin flounder Verasper moseri, reared in a white tank had a smaller ratio of pigmented area of the skin on non-eyed side, grew faster, and had greater melanin-concentrating hormone (MCH)-immunoreactive cell bodies and MCH gene expression in the brain than in the black tank, indicating that synthesis and release of MCH are higher in fish from a white tank. In the present study, a time-resolved fluoroimmunoassay for MCH was developed. MCH levels were assessed in the brain, pituitary gland, and plasma of barfin flounders reared in a white or black tank. A competitive assay using two antibodies was performed among secondary antibodies in the solid phase, MCH antibodies, samples, and europium-labeled MCH. Displacement curves of serially diluted extracts (brain, pituitary gland, and plasma) of the barfin flounder paralleled that of the MCH standard. MCH levels in the brain and plasma were higher in fish reared in the white tank for 5 months than in the black tank. These results suggest that synthesis and secretion of MCH are enhanced with the white background and that MCH is involved in both somatic growth and the skin pigmentation in the barfin flounder. (c) 2005 Elsevier Inc. All rights reserved.