A stele-enriched gene regulatory network in the Arabidopsis root
A stele-enriched gene regulatory network in the Arabidopsis root
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DOI：
10.1038/msb.2010.114
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发表时间：
2011-01-01
影响因子：
9.9
通讯作者：
Benfey, Philip N.
中科院分区：
文献类型：
--
作者：
Brady, Siobhan M.;Zhang, Lifang;Benfey, Philip N.
严格控制基因表达是多细胞发育的标志，由转录因子(TF)和microRNAs(MiRNAs)完成。虽然许多研究都集中在识别这些分子的下游靶点，但对调节它们差异表达的因素知之甚少。我们使用来自高空间分辨率基因表达实验以及酵母单杂交(Y1H)和双杂交(Y2H)的数据来描述在基因调控网络(GRN)中发生的相互作用的子集，该网络决定了植物中组织特异性的Tf和miRNA的表达。我们发现，上游转录因子在比其靶标更多样化的细胞类型中表达，并且被相对大量的转录因子结合的启动子对应于关键的发育调节因子。许多转铁蛋白对其靶标的调控结果是通过基因分析实验确定的。值得注意的是，65%的TF被鉴定出分子表型，但形态表型只有16%。这表明GRN是健壮的，基因表达的变化可能被引导或缓冲。《分子系统生物学》7：459；2011年1月18日在线出版；doi：10.1038/msb.2010.114
Tightly controlled gene expression is a hallmark of multicellular development and is accomplished by transcription factors (TFs) and microRNAs (miRNAs). Although many studies have focused on identifying downstream targets of these molecules, less is known about the factors that regulate their differential expression. We used data from high spatial resolution gene expression experiments and yeast one-hybrid (Y1H) and two-hybrid (Y2H) assays to delineate a subset of interactions occurring within a gene regulatory network (GRN) that determines tissue-specific TF and miRNA expression in plants. We find that upstream TFs are expressed in more diverse cell types than their targets and that promoters that are bound by a relatively large number of TFs correspond to key developmental regulators. The regulatory consequence of many TFs for their target was experimentally determined using genetic analysis. Remarkably, molecular phenotypes were identified for 65% of the TFs, but morphological phenotypes were associated with only 16%. This indicates that the GRN is robust, and that gene expression changes may be canalized or buffered. Molecular Systems Biology 7: 459; published online 18 January 2011; doi:10.1038/msb.2010.114