Emergence of Antagonism Against the Pathogenic Fungus Fusarium oxysporum by Interplay Among Non-Antagonistic Bacteria in a Hydroponics Using Multiple Parallel Mineralization
Emergence of Antagonism Against the Pathogenic Fungus Fusarium oxysporum by Interplay Among Non-Antagonistic Bacteria in a Hydroponics Using Multiple Parallel Mineralization
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DOI：
10.1111/jph.12504
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发表时间：
2016-12-01
影响因子：
1.5
通讯作者：
Shinohara, Makoto
中科院分区：
文献类型：
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作者：
Fujiwara, Kazuki;Iida, Yuichiro;Shinohara, Makoto
多重平行矿化(MPM)系统中的根际微生物群落有助于抑制根传疾病。我们假设这种现象可以归因于非拮抗细菌的相互作用，而不是单一的拮抗微生物。在这项研究中，我们通过研究MPM微生物群中细菌相互作用在抑制真菌植物病原体尖孢镰刀菌中的潜在作用来检验这一假设。对从MPM系统中分离到的细菌菌株进行了尖孢镰刀菌的体外和平板试验。一个由7个细菌菌株组成的群落(Kaistia sp.TbD58，鞘氨醇鞘氨醇sp.TbD84，Bosea sp.TBD101，链球杆菌属(Ancylobacter sp.TbD132、铜绿假单胞菌Cupriavidus sp.TbD162，短杆菌属。TbD179和Sphingopyxis sp.TBD181)抑制尖孢镰刀菌的生长。没有一株菌株单独对尖孢镰刀菌有拮抗作用，而几对非拮抗菌株对其生长有抑制作用。形态观察表明，在这些细菌对存在的情况下，可以形成肿胀的尖孢镰刀菌细胞。同样的细菌对也抑制了拟南芥的枯萎病。这些结果表明，在MPM系统中，非拮抗细菌之间复杂的细菌相互作用可以显著促进对尖孢镰刀菌拮抗作用的发展。
The rhizosphere microbial community in a multiple parallel mineralization (MPM) system contributes to suppression of root-borne diseases. We hypothesized this phenomenon can be attributed to the interplay of non-antagonistic bacteria rather than to a single antagonistic microbe. In this study, we tested this hypothesis by investigating the potential roles of bacterial interplay in a subset of MPM microbiota in the suppression of the fungal phytopathogen Fusarium oxysporum. Bacterial strains isolated from the MPM system were subjected to invitro and in planta tests on F.oxysporum. A community of seven bacterial strains (Kaistia sp. TBD58, Sphingopyxis sp. TBD84, Bosea sp. TBD101, Ancylobacter sp. TBD132, Cupriavidus sp. TBD162, Brevibacillus sp. TBD179 and Sphingopyxis sp. TBD181) suppressed F.oxysporum growth. None of the strains alone was antagonistic against F.oxysporum, whereas several pairs of those non-antagonistic strains inhibited its growth. Morphological observations showed the formation of swollen F.oxysporum cells in the presence of these bacterial pairs. The same bacterial pairs also suppressed Fusarium wilt disease in Arabidopsis thaliana. These results indicate that a complex bacterial interplay among non-antagonistic bacteria can significantly contribute to the development of antagonism against F.oxysporum in the context of the MPM system.