Forkhead box P3 is selectively expressed in human trophoblasts and decreased in recurrent pregnancy loss
Forkhead box P3 is selectively expressed in human trophoblasts and decreased in recurrent pregnancy loss
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叉头盒 P3 在人类滋养层细胞中选择性表达，并在复发性流产中减少
DOI：
10.1016/j.placenta.2019.04.003
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发表时间：
2019-06-01
期刊：
影响因子：
3.8
通讯作者：
Liao, Aihua
中科院分区：
文献类型：
--
作者：
Hu, Xiaohui;Wang, Yan;Liao, Aihua
引言：叉头盒P3（Foxp 3）是诱导调节性T细胞免疫抑制功能所必需的。最近的数据表明，一些非淋巴细胞，如某些肿瘤细胞，也表达Foxp 3，其参与肿瘤发生，并与肿瘤的侵袭和增殖行为有关。方法：采用免疫组织化学方法检测Foxp 3在早孕绒毛和蜕膜中的表达。采用流式细胞术检测Foxp 3在孕早期胎盘和足月胎盘中的表达水平。采用实时荧光定量PCR（RT-PCR）、免疫组化和Western blot方法检测Foxp 3在复发性流产（RPL）患者滋养细胞中的表达。Foxp 3在滋养层细胞柱和细胞滋养层的细胞核中表达。然而，在合胞体滋养层中观察到Foxp 3的低表达。Foxp 3在早孕蜕膜绒毛外滋养细胞中也有表达。足月胎盘滋养细胞Foxp 3表达较正常早孕明显降低。此外，Foxp 3的mRNA和蛋白水平下降，观察在妇女与RPL。讨论：Foxp 3在人滋养细胞的选择性表达表明，Foxp 3的表达可能与滋养细胞的增殖和侵袭行为。RPL患者滋养细胞Foxp 3表达降低可能有助于更好地了解RPL的病理特征。
Introduction: Forkhead box P3 (Foxp3) is necessary for induction of the immunosuppressive functions in regulatory T cells. Recent data indicate that some non-lymphoid cells, such as certain tumor cells, also express Foxp3, which participates in tumorigenesis and is related to the invasive and proliferative behavior of the tumor. However, the expression of Foxp3 in trophoblasts has not been studied previously.Methods: Localization of Foxp3 in the villi and decidua in the first trimester was determined using immunohistochemistry. Foxp3 expression was detected by flow cytometry, and the expression levels in the first trimester placenta were compared with those in the term placenta. Additionally, the Foxp3 expression in trophoblasts in recurrent pregnancy loss (RPL) was analyzed by real-time polymerase chain reaction (RT-PCR), immunohistochemistry and Western blot.Results: Foxp3 is expressed by trophoblasts in early pregnancy in the villi and decidua. Foxp3 is expressed in the nuclei of both trophoblast cell columns and cytotrophoblasts. However, low Foxp3 expression was observed in syncytiotrophoblasts. Foxp3 was also expressed by the extravillous trophoblasts in early pregnancy in decidua. The Foxp3 expression in trophoblasts in the term placenta was significantly decreased compared with that in the normal early pregnancy. Moreover, decreased levels of Foxp3 mRNA and protein were observed in women with RPL.Discussion: The selective expression of Foxp3 in human trophoblasts suggest that Foxp3 expression may be associated with the proliferation and invasion behavior of trophoblasts. The decreased Foxp3 expression in the trophoblasts in RPL may contribute to better understanding of the pathological characteristics of RPL.