Antimicrobial effect of an ultrasonic levitation washer disinfector with silver electrolysis and ozone oxidation on methicillin-resistant Staphylococcus aureus (MRSA).
Antimicrobial effect of an ultrasonic levitation washer disinfector with silver electrolysis and ozone oxidation on methicillin-resistant Staphylococcus aureus (MRSA).
复制标题
银电解和臭氧氧化超声波悬浮清洗消毒机对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌效果。
DOI：
10.1111/1346-8138.12327
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发表时间：
2013
期刊：
影响因子：
3.1
通讯作者：
Kanekura T.
中科院分区：
文献类型：
--
作者：
Tamai M;Matsushita S;Miyanohara H;Imuta N;Ikeda R;Kawai K;Nishi J;Sakamoto A;Shigihara T;Kanekura T.
耐甲氧西林金黄色葡萄球菌（MRSA）已迅速成为严重和顽固性皮肤感染的原因。目前，还没有有效的局部治疗方法，皮肤的MRSA感染或定殖引起了严重的医学问题。我们开发了一种超声波悬浮清洗机，可产生银离子（Ag+）和臭氧（O3），用于清洁和灭菌医疗器械。本文报道了Ag+和O_3超声悬浮对MRSA的体外和体内作用。在三种体外条件下检查了对六种同种型MRSA菌株的抗菌作用;细胞漂浮在水箱中，细胞浸润，细胞在去端肽胶原膜上形成生物膜。在体内研究中，我们分析了小鼠皮肤溃疡治疗后存活的MRSA微生物数量，并评估了溃疡大小。悬浮Ag+显着降低MRSA的生存漂浮在水箱中。用Ag+和O3悬浮显著降低了在去端肽胶原膜上浸润或形成生物膜的MRSA的活力，而不管生物膜的产生水平如何。体内研究表明，当连续7天进行15分钟的治疗时，小鼠皮肤溃疡上的MRSA数量显著减少，并且在第7个疗程后溃疡大小显著减小。用Ag+和O3悬浮可能是治疗皮肤MRSA感染和加速伤口愈合的有价值的工具。
Methicillin‐resistantStaphylococcus aureus(MRSA) has rapidly emerged as a cause of severe and intractable skin infection. At present, there are no effective topical treatments, and infection or colonization by MRSA of the skin raises serious medical problems. We developed an ultrasonic levitation washer that generates silver ions (Ag+) and ozone (O3) to clean and sterilize medical devices. We report the effect of ultrasonic levitation (levitation) with Ag+and O3on MRSAin vitroandin vivo. Antimicrobial effect against six MRSA strains of allagrtypes was examined under threein vitroconditions; cells floating in a water tank, cells infiltrating‐, and cells forming a biofilm on an atelocollagen membrane. In thein vivostudies, we assayed the number of MRSA organisms that survived treatment on murine skin ulcers and evaluated the ulcer size. Levitation with Ag+dramatically decreased the survival of MRSA floating in a water tank. Levitation with Ag+and O3significantly decreased the viability of MRSA that had infiltrated or formed a biofilm on atelocollagen membranes regardless of the level of biofilm production.In vivostudies showed that the number of MRSA on murine skin ulcers was significantly decreased when 15‐min treatment was performed for 7 consecutive days and that the ulcer size was significantly decreased after the seventh treatment course. Levitation with Ag+and O3may be a valuable tool for treating MRSA infestation of the skin and for accelerating wound healing.