Interferon activates promoter of Nmi gene via interferon regulator factor-1
Interferon activates promoter of Nmi gene via interferon regulator factor-1
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干扰素通过干扰素调节因子1激活Nmi基因启动子
DOI：
10.1007/s11010-017-3182-y
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发表时间：
2018-04-01
影响因子：
4.3
通讯作者：
Tan，Juan
中科院分区：
文献类型：
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作者：
Xu，Xiao;Chai，Keli;Tan，Juan
N-Myc相互作用子（Nmi）参与信号转导、转录调控和抗病毒反应等多种生物学功能。由于Nmi可能在干扰素（IFN）诱导的应答中起重要作用，我们研究了Nmi蛋白的调节机制。我们鉴定并克隆了Nmi基因的启动子。序列分析和荧光素酶分析表明，Nmi基因启动子中的一个干扰素刺激反应元件（ISRE）和一个GC盒对Nmi基因的基础转录活性是必需的。我们还发现干扰素调节因子1（IRF-1）可以通过与启动子中的ISRE结合来激活Nmi的转录。IRF-1的敲低降低IFN诱导的Nmi转录。这些结果表明IRF-1参与了IFN诱导的Nmi表达。
N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated. We identified and cloned the promoter of Nmi gene. Sequence analysis and luciferase assays shown that an IFN-stimulated response element (ISRE) and a GC box in the promoter were essential for the basal transcription activity of Nmi gene. We also found that interferon regulatory factor 1 (IRF-1) could activate transcription of Nmi by binding to the ISRE in the promoter. Knockdown of IRF-1 decreases IFN-induced Nmi transcription. These results revealed that IRF-1 is involved in the IFN-inducible expression of Nmi.