The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp lactis IL1403
The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp lactis IL1403
复制标题
DOI：
10.1101/gr.gr-1697r
复制
发表时间：
2001-05-01
期刊：
影响因子：
7
通讯作者：
Sorokin, A
中科院分区：
文献类型：
--
作者：
Bolotin, A;Wincker, P;Sorokin, A
乳酸乳球菌是一种非致病性的富含AT的革兰氏阳性细菌，与链球菌属密切相关，是最常用的奶酪发酵剂。它也是特征最好的乳酸菌。我们测序了实验室菌株IL 1403的基因组，使用一种新的两步策略，包括整个基因组的诊断测序和;鸟枪抛光步骤。该基因组包含2，365，589个碱基对，编码2310种蛋白质，其中包括属于6个原噬菌体的293个蛋白质编码基因和43个插入序列（IS）元件。IS元件的非随机分布表明，测序菌株的染色体可能是最近两个密切相关的基因组之间重组的产物。结果表明，该菌具有一套完整的晚期感受态基因。乳酸菌进行DNA转化。基因组序列揭示了发酵途径和有氧呼吸的新的可能性。这也表明遗传信息从乳球菌到沙门氏菌-埃希氏菌群的革兰氏阴性肠道细菌的水平转移。
Lactococcus lactis is a nonpathogenic AT-rich gram-positive bacterium closely related to the genus Streptococcus and is the most commonly used cheese starter. It is also the best-characterized lactic acid bacterium. We sequenced the genome of the laboratory strain IL1403, using a novel two-step strategy that comprises diagnostic sequencing of the entire genome and;a shotgun polishing step. The genome contains 2,365,589 base pairs and encodes 2310 proteins, including 293 protein-coding genes belonging to six prophages and 43 insertion sequence (IS) elements. Nonrandom distribution of IS elements indicates that the chromosome of the sequenced strain may be a product of recent recombination between two closely related genomes. A complete set of late competence genes is present, indicating the ability of L. lactis to undergo DNA transformation. Genomic sequence revealed new possibilities for fermentation pathways and for aerobic respiration. It also indicated a horizontal transfer of genetic information from Lactococcus to gram-negative enteric bacteria of Salmonella-Escherichia group.