Molecular approaches to the identification of Bulinus species in south-west Nigeria and observations on natural snail infections with schistosomes
Molecular approaches to the identification of Bulinus species in south-west Nigeria and observations on natural snail infections with schistosomes
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DOI：
10.1017/s0022149x10000568
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发表时间：
2011-09-01
影响因子：
1.6
通讯作者：
Adeleke, M. A.
中科院分区：
文献类型：
--
作者：
Akinwale, O. P.;Kane, R. A.;Adeleke, M. A.
目前的研究考虑了尼日利亚8个州内28个地点的138只Bulinus蜗牛的小样本分布。蜗牛进行了鉴定，使用分子方法的组合，包括部分细胞色素氧化酶亚基1（cox 1）片段的DNA测序和限制性内切酶（ITS）扩增产物。结果表明，所采集的泡螺样品绝大多数为截形泡螺，只有2个为球形泡螺。大多数样品采用Rsa Ⅰ限制性内切酶切割泡螺核糖体ITS作为种属鉴定的方法，这是一种更适合小型实验室环境的快速和廉价的方法。采用聚合酶链反应（PCR）扩增每个采集的泡螺样本内的Dra1重复序列，以确定样本区域内感染蜗牛的程度和分布。Dra1重复序列的成功扩增表明，29.7%的钉螺感染了螺旋体。从一个小的子集的蜗牛样本的部分染色体的测序表明，一些蜗牛要么穿透血吸虫和牛血吸虫毛蚴或杂交毛蚴形成的两个物种。
The current study considers the distribution of a small sample of 138 Bulinus snails, across 28 localities within eight Nigerian states. Snails were identified using a combination of molecular methods involving both DNA sequencing of a partial cytochrome oxidase subunit 1 (cox1) fragment and restriction profiles obtained from ribosomal internal transcribed spacer (its) amplicons. The results showed that the majority of Bulinus samples tested belonged to the species Bulinus truncatus while only two were Bulinus globosus. The use of Rsa I restriction endonuclease to cleave the ribosomal its of Bulinus, as a method of species identification, was adopted for the majority of samples, this being a quicker and cheaper method better suited to small laboratory environments. Polymerase chain reaction (PCR) amplification of the schistosome Dra1 repeat within each of the collected Bulinus samples was employed to determine the extent and distribution of infected snails within the sample areas. Successful amplification of the Dra1 repeat demonstrated that 29.7% of snails were infected with schistosomes. Sequencing of the partial schistosome its from a small subset of snail samples suggested that some snails were either penetrated by both Schistosoma haematobium and Schistosoma bovis miracidia or hybrid miracidia formed from the two species.