Dysferlin is essential for endocytosis in the sea star oocyte.
Dysferlin is essential for endocytosis in the sea star oocyte.
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Dysferlin 对于海星卵母细胞的内吞作用至关重要。
DOI：
10.1016/j.ydbio.2013.12.018
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发表时间：
2014
影响因子：
2.7
通讯作者：
Wessel,GaryM
中科院分区：
文献类型：
--
作者：
Oulhen,Nathalie;Onorato,ThomasM;Ramos,Isabela;Wessel,GaryM
Dysferlin 是一种钙结合跨膜蛋白，参与膜融合和膜修复。在人类中，dysferlin 基因的突变与肌营养不良症有关。在这项研究中，我们从海星成熟的未成熟卵母细胞中分离出富含质膜的部分，并通过质谱分析鉴定了 Dysferlin。全长 Dysferlin 序列在人类和海星之间高度保守。我们了解到，在海星Patiria miniata中，dysferlinRNA和蛋白质在卵子发生到原肠胚形成过程中都有表达。有趣的是，该蛋白质在卵母细胞质膜中高度富集。注射吗啉代抗dysferlin会导致卵母细胞内吞作用减少，并导致原肠胚形成过程中的发育停滞。这些结果表明，dysferlin 对于卵子发生过程中的正常内吞作用和海星的胚胎发生至关重要，并且该动物可能是研究dysferlin 结构与其功能关系的有用模型。
Dysferlin is a calcium-binding transmembrane protein involved in membrane fusion and membrane repair. In humans, mutations in the dysferlin gene are associated with muscular dystrophy. In this study, we isolated plasma membrane-enriched fractions from full-grown immature oocytes of the sea star, and identified dysferlin by mass spectrometry analysis. The full-length dysferlin sequence is highly conserved between human and the sea star. We learned that in the sea starPatiria miniata,dysferlinRNA and protein are expressed from oogenesis to gastrulation. Interestingly, the protein is highly enriched in the plasma membrane of oocytes. Injection of a morpholino against dysferlin leads to a decrease of endocytosis in oocytes, and to a developmental arrest during gastrulation. These results suggest that dysferlin is critical for normal endocytosis during oogenesis and for embryogenesis in the sea star and that this animal may be a useful model for studying the relationship of dysferlin structure as it relates to its function.