A rapid and inexpensive method to screen for common foods that reduce the action of acrylamide, a harmful substance in food
A rapid and inexpensive method to screen for common foods that reduce the action of acrylamide, a harmful substance in food
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DOI：
10.1016/j.toxlet.2007.09.013
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发表时间：
2007-12-10
影响因子：
3.5
通讯作者：
Miwa, Johji
中科院分区：
文献类型：
--
作者：
Hasegawa, Koichi;Miwa, Satsuki;Miwa, Johji
通过DNA微阵列和蛋白质2-DE筛选丙烯酰胺上调的秀丽隐杆线虫基因，我们选择了gst-4基因并构建了gst::gfp融合基因，用于将秀丽隐杆线虫转化为丙烯酰胺生物传感器。该生物传感器以剂量和时间依赖性方式将丙烯酰胺检测为 GFP 表达信号。当生物传感器与市售绿茶粉一起暴露于丙烯酰胺时，GFP 水平下降至对照水平，表明丙烯酰胺解毒或防止 GST 诱导。本方法不仅适用于筛选有害物质，还适用于通过适当构建的可见生物传感器筛选减少或抵消其危害性或作用的物质。 (C) 2007 Elsevier Ireland Ltd. 保留所有权利。
By DNA microarray and protein 2-DE screens for Caenorhabditis elegans genes up-regulated by acrylamide, we selected the gst-4 gene and constructed a gst::gfp fusion gene, which was used to transform C. elegans into a biosensor for acrylamide. This biosensor detects acrylamide as a GFP-expression signal in a dose- and time-dependent manner. When the biosensor was exposed to acrylamide together with commercially available powdered green tea, GFP levels decreased to the control level, suggestive of acrylamide detoxification or prevention of GST induction. The present methodology should be applicable for screening of not only harmful substances but also substances that reduce or counteract their harmfulness or action, with appropriately constructed visible biosensors. (C) 2007 Elsevier Ireland Ltd. All rights reserved.