Plastic fiber optics for micro-imaging of fluorescence signals in living cells
Plastic fiber optics for micro-imaging of fluorescence signals in living cells
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DOI：
10.1117/12.2076928
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发表时间：
2015-03
期刊：
影响因子：
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通讯作者：
T. Sakurai;M. Natsume;K. Koida
中科院分区：
文献类型：
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作者：
T. Sakurai;M. Natsume;K. Koida
光纤耦合显微镜（FCM）能够在其他光学技术无法到达的组织或器官的深层部位进行体内成像。为了开发基于FCM的活体成像，我们采用了塑料光纤（POF）束，其中包括超过10，000个单位的聚苯乙烯芯和聚甲基丙烯酸甲酯包层。每个POF的直径小于5 μm;束的尖端宽度小于0.5 mm，柔性线的长度为1，000 mm。塑料FCM的光学性能足以检测用钙离子敏感荧光染料标记的大鼠胰腺腺泡中的显著信号变化。在未来，塑料FCM的潜力预计将增加，使脆弱器官内活细胞的结构和组织的特定功能的分析。
The fiber-coupled microscope (FCM) enables in vivo imaging at deep sites in the tissues or organs that other optical techniques are unable to reach. To develop FCM-based intravital imaging, we employed a plastic optical fiber (POF) bundle that included more than 10,000-units of polystyrene core and polymethyl methacrylate cladding. Each POF had a diameter of less than 5 μm; the tip of the bundle was less than 0.5 mm wide, and the flexible wire had a length of 1,000 mm. The optical performance of the plastic FCM was sufficient for detection of significant signal changes in an acinus of rat pancreas labeled with a calcium ion–sensitive fluorescent dye. In the future, the potential power of plastic FCM is expected to increase, enabling analysis of structure and organization of specific functions in live cells within vulnerable organs.