Effects of bradykinin on DNA synthesis in resting NIL8 hamster cells and human fibroblasts.
Effects of bradykinin on DNA synthesis in resting NIL8 hamster cells and human fibroblasts.
复制标题
缓激肽对静息 NIL8 仓鼠细胞和人成纤维细胞 DNA 合成的影响。
DOI：
10.1016/0014-4827(84)90358-6
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发表时间：
1984
影响因子：
3.7
通讯作者：
Pang,KJ
中科院分区：
文献类型：
--
作者：
Straus,DS;Pang,KJ
缓激肽刺激[3 H]胸苷掺入和DNA合成在休息，血清剥夺NIL 8仓鼠细胞。该刺激的ED 50为4.52 ± 2.91 nM。其他激肽肽包括赖氨酸-缓激肽（胰激肽）和甲硫氨酸-赖氨酸-缓激肽也刺激[3 H]胸苷掺入NIL 8细胞，而desarg 9-缓激肽没有效果，表明激肽肽通过B2型受体的作用。缓激肽也刺激IMR-90人成纤维细胞中的DNA合成;然而，仅在存在吲哚美辛的情况下观察到这种作用，吲哚美辛阻断前列腺素合成。这些结果表明，在成纤维细胞缓激肽的生长刺激作用的负调制器中的蜀葵素。外源性PGE 1、PGE 2、PGA 1、PGA 2、PGB 1和PGB 2可明显抑制人成纤维细胞[3 H]胸苷掺入。在NIL 8细胞中观察到的缓激肽的直接作用可能归因于这些细胞对洋地黄素的生长抑制的相对抗性。
Bradykinin stimulates [3H]thymidine incorporation and DNA synthesis in resting, serum-deprived NIL8 hamster cells. The ED50for this stimulation is 4.52 ± 2.91 nM. Other kinin peptides including lys-bradykinin (kallidin) and met-lys-bradykinin also stimulate [3H]thymidine incorporation in the NIL8 cells, whereas desarg9-bradykinin is without effect, suggesting action of the kinin peptides through type B2receptors. Bradykinin also stimulates DNA synthesis in IMR-90 human fibroblasts; however, this effect is observed only in the presence of indomethacin, which blocks prostaglandin synthesis. These results suggest that prostaglandins act as negative modulators of the growth-stimulatory effects of bradykinin in the fibroblasts. This conclusion is supported by the observation that exogenously added PGE1, PGE2, PGA1, PGA2, PGB1, and PGB2strongly inhibit [3H]thymidine incorporation in the human fibroblasts. The direct effect of bradykinin observed in the NIL8 cells may be attributable to the relative resistance of these cells to growth inhibition by prostaglandins.