Rice OsPEX1, an extensin-like protein, affects lignin biosynthesis and plant growth
Rice OsPEX1, an extensin-like protein, affects lignin biosynthesis and plant growth
复制标题
水稻 OsPEX1 是一种类延伸蛋白，影响木质素生物合成和植物生长
DOI：
10.1007/s11103-019-00849-3
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发表时间：
2019-05-01
影响因子：
5.1
通讯作者：
Zhang, Xiang-Qian
中科院分区：
文献类型：
--
作者：
Ke, Shanwen;Luan, Xin;Zhang, Xiang-Qian
关键信息水稻富含亮氨酸的重复延伸蛋白样蛋白 OsPEX1 介导木质素沉积和植物生长的交叉。 摘要木质素是次生细胞壁的主要结构成分，对于正常植物生长和发育至关重要。然而，水稻木质素生物合成的分子和遗传调控尚未完全了解。在此，我们报告了具有硬秆的水稻半显性矮化突变体（pex1）的鉴定和表征。分子和遗传分析表明，pex1 表型是由富含亮氨酸的重复延伸样基因 OsPEX1 的异位表达引起的。有趣的是，与野生型植物相比，thepex1突变体显示出显着更高的木质素含量和木质素相关基因的表达水平增加。相反，OsPEX1抑制的转基因表现出低木质素含量和与木质素生物合成相关的基因的转录丰度降低，表明OsPEX1介导水稻中的木质素生物合成和/或沉积。当OsPEX1在缺乏众所周知的绿色革命基因半矮化1等位基因的高秆水稻品种中异位表达时，所得转基因植株表现出高度降低和抗倒伏性增强。我们的研究揭示了 OsPEX1 的表达与木质素沉积之间的因果关系。最后，我们证明调节OsPEX1表达可以为提高水稻抗倒伏性提供工具。
Key messageRice leucine-rich repeat extensin-like protein OsPEX1 mediates the intersection of lignin deposition and plant growth.AbstractLignin, a major structural component of secondary cell wall, is essential for normal plant growth and development. However, the molecular and genetic regulation of lignin biosynthesis is not fully understood in rice. Here we report the identification and characterization of a rice semi-dominant dwarf mutant (pex1) with stiff culm. Molecular and genetic analyses revealed that thepex1phenotype was caused by ectopic expression of a leucine-rich repeat extension-like gene,OsPEX1. Interestingly, thepex1mutant showed significantly higher lignin content and increased expression levels of lignin-related genes compared with wild type plants. Conversely,OsPEX1-suppresssed transgenics displayed low lignin content and reduced transcriptional abundance of genes associated with lignin biosynthesis, indicating that theOsPEX1mediates lignin biosynthesis and/or deposition in rice. WhenOsPEX1was ectopically expressed in rice cultivars with tall stature that lacks the allele ofsemi-dwarf 1, well-known green revolution gene, the resulting transgenic plants displayed reduced height and enhanced lodging resistance. Our study uncovers a causative effect between the expression ofOsPEX1and lignin deposition. Lastly, we demonstrated that modulatingOsPEX1expression could provide a tool for improving rice lodging resistance.