WFS1 protein modulates the free Ca2+ concentration in the endoplasmic reticulum
WFS1 protein modulates the free Ca2+ concentration in the endoplasmic reticulum
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DOI：
10.1016/j.febslet.2006.09.007
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发表时间：
2006-10-16
期刊：
影响因子：
3.5
通讯作者：
Oka, Yoshitomo
中科院分区：
文献类型：
--
作者：
Takei, Daisuke;Ishihara, Hisamitsu;Oka, Yoshitomo
WFS 1基因编码内质网（ER）膜糖蛋白，在以糖尿病和视神经萎缩为特征的Wolfram综合征中发生突变。在此，在WFS 1敲低和过量表达的HEK 293细胞中检查Ca 2+动力学。使用ER靶向的Ca 2+敏感性发光蛋白水母发光蛋白的研究表明，WFS 1蛋白通过增加Ca 2+摄取速率来正向调节ER Ca 2+水平。此外，Fura-2的Ca 2+成像显示了钙库操作的Ca 2+进入平行WFS 1表达水平的幅度。这些数据表明，WFS 1蛋白参与细胞内钙稳态的调节，至少部分地，通过调节ER钙库的填充状态。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The WFS1 gene, encoding an endoplasmic reticulum (ER) membrane glycoprotein, is mutated in Wolfram syndrome characterized by diabetes mellitus and optic atrophy. Herein, Ca2+ dynamics were examined in WFS1-knockdown and -over-expressing HEK293 cells. Studies using ER-targeted Ca2+ -sensitive photoprotein aequorin demonstrated WFS1 protein to positively modulate ER Ca2+ levels by increasing the rate of Ca2+ uptake. Furthermore, Ca2+ imaging with Fura-2 showed the magnitude of the store-operated Ca2+ entry to parallel WFS1 expression levels. These data indicate that WFS1 protein participates in the regulation of cellular Ca2+ homeostasis, at least partly, by modulating the filling state of the ER Ca2+ store. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.