Poly(A) RNA in Bacillus subtilis: identification of the polyadenylylation site of flagellin mRNA.
Poly(A) RNA in Bacillus subtilis: identification of the polyadenylylation site of flagellin mRNA.
复制标题
枯草芽孢杆菌中的 Poly(A) RNA：鞭毛蛋白 mRNA 聚腺苷酸化位点的鉴定。
DOI：
10.1111/j.1574-6968.1993.tb06116.x
复制
发表时间：
1993
影响因子：
2.1
通讯作者：
Sarkar,N
中科院分区：
文献类型：
--
作者：
Cao,GJ;Sarkar,N
使用改进的合成、扩增和克隆与细菌poly(A) RNA互补的DNA的方法，我们成功地推导出由枯草芽孢杆菌haggene编码的聚腺苷酸化鞭毛蛋白mRNA的3'端序列。发现聚腺苷酸化位点正好位于对应于hag基因的推定的rho独立转录终止子的末端茎环结构的上游。这对应于大肠杆菌脂蛋白 (lpp) mRNA 的主要聚腺苷酸化位点 [G.C.高和 N.萨卡 (1992) Proc。国家。阿卡德。科学。 USA 89, 7546–7550]，表明在革兰氏阳性菌和革兰氏阴性菌中，初级转录物在多聚腺苷酸化之前通过核酸内切裂解进行加工。
Using improved procedures for the synthesis, amplification, and cloning of DNA complementary to bacterial poly(A) RNA, we succeeded in deducing the 3′-terminal sequence of polyadenylylated flagellin mRNA encoded by theBacillus subtilis haggene. The site of polyadenylylation was found to be just upstream of the terminal stem-loop structure corresponding to the putative rho-independent transcription terminator of thehaggene. This corresponds to the major polyadenylation site ofEscherichia colilipoprotein (lpp) mRNA [G.C. Gao and N. Sarkar (1992) Proc. Natl. Acad. Sci. USA 89, 7546–7550], suggesting that the primary transcript is processed by endonucleolytic cleavage prior to polyadenylylation in Gram-positive as well as in Gram-negative bacteria.