Isolation and expression analysis of Salt Overly Sensitive gene family in grapevine (Vitisvinifera) in response to salt and PEG stress
Isolation and expression analysis of Salt Overly Sensitive gene family in grapevine (Vitisvinifera) in response to salt and PEG stress
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葡萄（Vitisvinifera）响应盐和 PEG 胁迫的盐过度敏感基因家族的分离和表达分析
DOI：
10.1371/journal.pone.0212666
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发表时间：
2019-03-19
期刊：
影响因子：
3.7
通讯作者：
Cheng, Zong-Ming (Max)
中科院分区：
文献类型：
--
作者：
Ma, Yuanchun;Wang, Li;Cheng, Zong-Ming (Max)
盐胁迫是影响葡萄生产和产量的主要环境因素之一。SOS3基因家族是盐过度敏感（SOS）信号通路的一部分，这是一个明确的信号通路，已知在植物对盐胁迫的反应中起作用。本研究对葡萄SOS3基因家族进行了标注，并对标注基因在盐胁迫响应中的作用进行了表征。在葡萄基因组中鉴定出9个葡萄SOS3基因，并对其进行分析。反转录定量PCR （RT-qPCR）检测了9个VviSOS3基因在离体黑皮诺葡萄品种（PN40024）叶片、根和茎中对盐（250mM NaCl）和聚乙二醇6000 （PEG，渗透压与盐处理相同）处理36h后的表达谱变化很大。除VviSOS3.7基因外，其余VviSOS3基因在盐和PEG处理下均表达上调。除VviSOS3.8外，大部分VviSOS3基因在PEG胁迫下在根中表达上调，而在盐胁迫下在根和茎中表达相反。盐度处理降低了可溶性蛋白含量。基于表达模式和生理数据，我们确定了VviSOS3.7和VviSOS3.8作为候选基因，对其在葡萄对盐胁迫响应中的作用进行进一步的功能表征。
Salt stress is one of the major environmental constraints for the production and yield of grape (Vitis vinifera) worldwide. The SOS3 gene family is part of the Salt Overly Sensitive (SOS) signaling pathway, a well-defined signaling pathway known to play a role in plant response to salt stress. In this study, the grapevine SOS3 gene family was annotated and the role of the annotated genes in salinity stress response was characterized. Nine grapevine SOS3 genes was identified in the grapevine genome and was subsequently analyzed. The expression patterns of the nine VviSOS3 genes, as determined by reverse transcription quantitative PCR (RT-qPCR), varied greatly in leaves, roots, and stems of in-vitro grown Pinot noir grapevine cultivar(PN40024) in response to salt (250mM NaCl) and polyethylene glycol 6000 (PEG, osmolality equal to the salt treatment) treatments over a 36h time period. All of the VviSOS3 genes, except VviSOS3.7, were up-regulated in leaves in response to the salt and PEG treatments. The majority of VviSOS3 genes, except VviSOS3.8 were up-regulated in roots in response to the PEG stress, with an opposite expression pattern in the root and stem in response to salt stress. The salinity treatment decreased the soluble protein content. Based on the expression pattern and physiological data, VviSOS3.7 and VviSOS3.8 were identified as candidate genes for further functional characterizations regarding their role in the response of grapevine to salt stress.