Phagocytosis by neutrophils induces an amino acid deprivation response in Saccharomyces cerevisiae and Candida albicans
Phagocytosis by neutrophils induces an amino acid deprivation response in Saccharomyces cerevisiae and Candida albicans
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DOI：
10.1073/pnas.1834481100
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发表时间：
2003-09-16
影响因子：
11.1
通讯作者：
Fink, GR
中科院分区：
文献类型：
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作者：
Rubin-Bejerano, I;Fraser, I;Fink, GR
通过使用全基因组阵列比较了被人类中性粒细胞或单核细胞吞噬的酵母细胞的转录谱。中性粒细胞吞噬后，酿酒酵母和白色念珠菌都通过诱导蛋氨酸和精氨酸途径的基因做出反应。这些途径均不是在单核细胞吞噬作用时诱导的。当从富含氨基酸的培养基转移到缺乏氨基酸的培养基时，两种真菌表现出类似的这些途径的诱导。这些数据表明中性粒细胞的内部吞噬体是一个缺乏氨基酸的环境。
The transcriptional profiles of yeast cells that have been phagocytosed by either human neutrophils or monocytes were compared by using whole genome arrays. After phagocytosis by neutrophils, both Saccharomyces cerevisiae and Candida albicans respond by inducing genes of the methionine and arginine pathways. Neither of these pathways is induced upon phagocytosis by monocytes. Both fungi show a similar induction of these pathways when transferred from amino acid-rich medium to amino acid-deficient medium. These data suggest that the internal phagosome of the neutrophil is an amino acid-deficient environment.