Interleukin-15 and Interleukin-7 are the Major Cytokines to Maintain Endometriosis
Interleukin-15 and Interleukin-7 are the Major Cytokines to Maintain Endometriosis
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DOI：
10.1159/000496607
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发表时间：
2019-09-01
影响因子：
2.1
通讯作者：
Podgaec, Sergio
中科院分区：
文献类型：
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作者：
Bellelis, Patrick;Barbeiro, Denise Frediani;Podgaec, Sergio
目的：本研究的目的是评估细胞因子相关的自然杀伤细胞和调节性T细胞在子宫内膜异位症的病变，腹腔液（PF）和外周血（PB）的深浸润型子宫内膜异位症患者。研究设计：在一家三级转诊医院进行病例对照研究。将64例腹腔镜术后患者分为2组：子宫内膜异位症（A组- n = 32）和无子宫内膜异位症（B组- n = 32）。主要结局指标：使用Luminex（TM）多重悬浮珠阵列测量白细胞介素（IL）-2、IL-4、IL-7、IL-10、IL-12、IL-15、转化生长因子β 1和IFN γ浓度。评价了子宫内膜异位症患者的异位病灶和在位子宫内膜组织，以及所有患者的PF和PB。结果如下：与其他分析组相比，子宫内膜异位症组异位内膜中IL-15浓度显著较高，在位内膜中IL-7浓度显著较高（p < 0.05）。对照组PF中IFN γ、IL-7、IL-15水平明显高于子宫内膜异位症组，IL-10水平低于子宫内膜异位症组（p < 0.05）。在PB中，与子宫内膜异位症组相比，对照组中的IL-4、IL-10、IL-12、IL-15和IFN γ浓度显著升高（p < 0.05）。结论：我们的假设是深部子宫内膜异位症是一种失控的疾病。这种疾病的性质是邻近结构的进展和侵袭，并且这种疾病状态的证据是细胞因子调节和炎症的紊乱分泌，这似乎是负责维持疾病的因素之一。
Objective: The objective of this study was to evaluate cytokines related to natural killer and T-regulatory cells in endometriotic lesions, peritoneal fluid (PF) and the peripheral blood (PB) of patients with deep infiltrative endometriosis. Study Design: A case-control study was conducted in a tertiary referral hospital. Sixty-four consecutive patients after laparoscopy were divided into 2 groups: with endometriosis (Group A - n = 32) and without endometriosis (Group B - n = 32). Main Outcome Measures: Interleukin (IL)-2, IL-4, IL-7, IL-10, IL-12, IL-15, transforming growth factor beta 1, and IFN gamma concentration was measured using a Luminex(TM) multiplex suspension bead array. Tissues from endometriotic lesions of patients with endometriosis and from eutopic endometrium were evaluated, as well as PF and PB of all patients. Results: Compared to the other analyzed groups, IL-15 concentration was significantly higher in the ectopic endometrium and IL-7 in the eutopic endometrium of the endometriosis group (p < 0.05). Compared to endometriosis group, IFN gamma, IL-7, and IL-15 were observed to be significantly higher in the PF of the control group, and IL-10 was lower in the control group (p < 0.05). In PB, compared to endometriosis group, IL-4, IL-10, IL-12, IL-15, and IFN gamma concentrations were significantly higher in the control group (p < 0.05). Conclusions: Our hypothesis is that deep endometriosis is a disease out of control. This disease's nature is of progression and invasion of adjacent structures, and proof of this disease state is the disorganized secretion of cytokine regulation and inflammation, which seem to be among the factors responsible for the maintenance of the disease.