Serine protease inhibitors block invasion of host cells by Toxoplasma gondii
Serine protease inhibitors block invasion of host cells by Toxoplasma gondii
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DOI：
10.1128/aac.43.6.1358
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发表时间：
1999-06-01
影响因子：
4.9
通讯作者：
Dubremetz, JF
中科院分区：
文献类型：
--
作者：
Conseil, V;Soête, M;Dubremetz, JF
我们研究了蛋白酶抑制剂对原生动物寄生虫刚地弓形虫无性发育的影响。在测试的抑制剂中，只有两种不可逆的丝氨酸蛋白酶抑制剂，即3,4 - 二氯异香豆素和4 -（2 - 氨乙基） - 苯磺酰氟，通过以剂量依赖的方式特异性地影响寄生虫靶点，明显阻止了宿主细胞的入侵，其半数抑制浓度分别在1到5微摩尔和50到100微摩尔之间。在已证明抑制入侵的实验条件范围内，这两种化合物都没有显著影响寄生虫的形态、基本代谢或滑行运动。没有观察到部分入侵现象，这意味着抑制作用发生在相互作用的早期阶段。这些结果表明，该寄生虫至少有一种丝氨酸蛋白酶参与了刚地弓形虫的入侵过程。
We investigated the effect of protease inhibitors on the asexual development of the protozoan parasite Toxoplasma gondii. Among the inhibitors tested only mo irreversible serine protease inhibitors, 3,4-dichloroisocoumarin and 4-(2-aminoethyl)-benzenesulfonyl fluoride, clearly prevented invasion of the host cells by specifically affecting parasite targets in a dose-dependent manner, with 50% inhibitory concentrations between 1 and 5 and 50 and 100 mu M, respectively, Neither compound significantly affected parasite morphology basic metabolism, or gliding motility within the range of the experimental conditions in which inhibition of invasion was demonstrated. No partial invasion was observed, meaning that inhibition occurred at an early stage of the interaction. These results suggest that at least one serine protease of the parasite is involved in the invasive process of T. gondii.