Reinforcement of injectable calcium phosphate cement by gelatinized starches
Reinforcement of injectable calcium phosphate cement by gelatinized starches
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DOI：
10.1002/jbm.b.33434
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发表时间：
2016-04-01
影响因子：
3.4
通讯作者：
Yang, Lei
中科院分区：
文献类型：
--
作者：
Liu, Huiling;Guan, Ying;Yang, Lei
目前可注射磷酸钙骨水泥（CPC）存在强度低、脆性大、在水环境中凝聚力低等问题，严重阻碍了其在承重骨替代和微创骨科手术中的临床应用。在这里，使用糊化淀粉增强可注射CPC的策略进行了研究。四种类型的淀粉，即玉米淀粉，交联淀粉，阳离子淀粉，和钙改性淀粉，研究了它们对CPC的机械性能，注射性，凝固时间，抗崩解性和细胞相容性的影响。糊化淀粉在不同程度上显著提高了CPC的抗压强度、抗压模量和应变能密度。具体而言，玉米淀粉和钙改性淀粉显示六倍和两倍以上的CPC的抗压强度和模量的增加，分别。适量糊化淀粉的加入可提高CPC的注射性能和抗崩解性能。此外，对改性水泥浸出液的成骨细胞增殖试验表明，糊化淀粉对细胞增殖没有不利影响，与磷酸盐缓冲溶液对照相比，所有水泥样品均导致更好的成骨细胞增殖。初步探讨了不同淀粉的增强机理。根据淀粉zeta电位和粘度、水泥微观结构和所得力学性能的结果，提出了两种可能的机制，即由第二相的糊化淀粉和磷灰石晶体的强互锁增强。总之，加入糊化淀粉可以是一种有效的，简便的，生物友好的策略，以加强可注射CPC和提高其机械稳定性，因此，应进一步研究和开发。(c)2015 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，104 B：615-625，2016。
Current injectable calcium phosphate bone cements (CPC) encounter the problems of low strength, high brittleness, and low cohesion in aqueous environment, which greatly hinder their clinical applications for loading-bearing bone substitution and minimally invasive orthopedic surgeries. Here, a strategy of using gelatinized starches to reinforce injectable CPC was investigated. Four types of starches, namely corn starch, crosslinked starch, cationic starch, and Ca-modified starch, were studied for their influence on CPC mechanical properties, injectability, setting times, anticollapsibility, and cytocompatibility. Gelatinized starch significantly improved compressive strength and modulus as well as strain energy density of CPC to different extents. Specifically, both corn starch and Ca-modified starch revealed sixfold and more than twofold increases in the compressive strength and modulus of CPC, respectively. The addition of gelatinized starches with proper contents increased the injectability and anticollapsibility of CPC. In addition, osteoblast proliferation tests on leaching solution of modified cements showed that gelatinized starches had no adverse effect on cell proliferation, and all cement samples resulted in better osteoblast proliferation compared to phosphate-buffered solution control. The mechanisms behind the reinforcing effect of different starches were preliminarily studied. Two possible mechanisms, reinforcement by the second phase of gelatinized starch and strong interlocking of apatite crystals, were proposed based on the results of starch zeta potential and viscosity, cement microstructure, and resultant mechanical properties. In conclusion, incorporating gelatinized starches could be an effective, facile, and bio-friendly strategy to reinforce injectable CPC and improve its mechanical stability, and thus, should be further studied and developed. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 615-625, 2016.