In Vitro and In Vivo Evaluation of Lactobacillus delbrueckii subsp. bulgaricus KLDS1.0207 for the Alleviative Effect on Lead Toxicity.
In Vitro and In Vivo Evaluation of Lactobacillus delbrueckii subsp. bulgaricus KLDS1.0207 for the Alleviative Effect on Lead Toxicity.
复制标题
德氏乳杆菌保加利亚亚种KLDS1.0207缓解铅毒性作用的体外和体内评价
作者：
Li B;Jin D;Yu S;Etareri Evivie S;Muhammad Z;Huo G;Liu F
铅（Pb）是一种有毒的重金属污染物，可对人类和动物造成各种危害。在本研究中，乳酸杆菌德氏亚种。保加利亚乳杆菌KLDS1.0207（L.保加利亚种KLDS1.0207）具有较强的铅结合能力和较强的耐铅能力。结果表明，L.保加利亚小球藻KLDS1.0207与Pb的结合分别符合Langmuir-Freundlich模型和准二级动力学模型。扫描电子显微镜和能谱分析表明，细胞表面被铅覆盖，碳和氧元素主要参与铅的结合。结合傅立叶变换红外光谱分析，发现羧基、磷酰基、羟基、氨基和酰胺基是参与吸附的主要官能团。L.保加利亚乳杆菌KLDS1.0207对小鼠急性铅中毒的预防和治疗作用。保加利亚小白鼠KLDS1.0207处理可降低死亡率，有效提高粪铅水平，减轻组织铅富集，提高肝脏和肾脏抗氧化指标，减轻肾脏病理损伤。结果表明，L.保加利亚KLDS1.0207可作为一种潜在的抗急性铅中毒的益生菌。
Lead (Pb) is a toxic contaminating heavy metal that can cause a variety of hazardous effects to both humans and animals. In the present study, Lactobacillus delbrueckii subsp. bulgaricus KLDS1.0207 (L. bulgaricus KLDS1.0207), which has a remarkable Pb binding capacity and Pb tolerance, was selected for further study. It was observed that the thermodynamic and kinetic model of L. bulgaricus KLDS1.0207 Pb binding respectively fit with the Langmuir–Freundlich model and the pseudo second-order kinetic model. Scanning electron microscopy and energy dispersive spectroscopy analysis disclosed that the cell surfaces were covered with Pb and that carbon and oxygen elements were chiefly involved in Pb binding. Combined with Fourier transform infrared spectroscopy analysis, it was revealed that the carboxyl, phosphoryl, hydroxyl, amino and amide groups were the main functional groups involved in the Pb adsorption. The protective effects of L. bulgaricus KLDS1.0207 against acute Pb toxicity in mice was evaluated by prevention and therapy groups, the results in vivo showed that L. bulgaricus KLDS1.0207 treatment could reduce mortality rates, effectively increase Pb levels in the feces, alleviate tissue Pb enrichment, improve the antioxidant index in the liver and kidney, and relieve renal pathological damage. Our findings show that L. bulgaricus KLDS1.0207 can be used as a potential probiotic against acute Pb toxicity.