Replacing the Mixed Function Oxidase N-Demethylation by Hydrogen Peroxide and either Hemoglobin or Ferrous Sulfate
Replacing the Mixed Function Oxidase N-Demethylation by Hydrogen Peroxide and either Hemoglobin or Ferrous Sulfate
复制标题
用过氧化氢和血红蛋白或硫酸亚铁替代混合功能氧化酶 N-去甲基化
作者：
S. Zbaida;D. Gilhar;J. Silman
从n -去甲基化氨基吡啶、n -甲基哌嗪和光学活性（+）和(-)-麻黄碱的孵育混合物中以及从过氧化氢与血红蛋白或硫酸亚铁的反应中释放的甲醛被定量。立体选择性仅在肝微粒体孵育中观察到。从N-乙基吗啡啉、氨基吡啶、N-甲基哌嗪和N，N ' -二甲基哌嗪与硫酸亚铁和过氧化氢（Fenton试剂）反应中分离得到N-甲酰基衍生物。吸电子基团（NH, NCH3， O）在n-烷的第4位！用Fenton试剂催化n -脱烷基。
Formaldehyde liberated from N-demethylation of aminopyrine, N-methyl-piperazine and from the optically active (+)-and (–)-ephedrine was quantitated from the incubation mixtures of hepatic microsomes and from reactions of hydrogen peroxide with either hemoglobin or ferrous sulfate. Stereoselectivity was observed only in incubations with hepatic microsomes. N-Formyl derivatives were isolated from the reactions of N-ethylmorpholine, aminopyrine, N-methylpiperazine and N,N′-dimethyl-piperazine with ferrous sulfate and hydrogen peroxide (Fenton reagent). Electron-withdrawing groups (NH, NCH3, O) at the fourth position to the N-alky! group facilitate N-dealkylation with Fenton reagent.