Expression, nuclear localization and interactions of human MCM/P1 proteins.
Expression, nuclear localization and interactions of human MCM/P1 proteins.
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DOI：
10.1006/bbrc.1997.6865
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发表时间：
1997-07
影响因子：
3.1
通讯作者：
H. Tsuruga;N. Yabuta;Katsuhito Hashizume;Masako Ikeda;Yuichi Endo;Hiroshi Nojima
中科院分区：
文献类型：
--
作者：
H. Tsuruga;N. Yabuta;Katsuhito Hashizume;Masako Ikeda;Yuichi Endo;Hiroshi Nojima
我们在此报告了人类Mcm/P1蛋白（HsMcm2, -3， -5和-7）的比较分析，包括它们在细胞周期和静止状态下的相互作用，细胞周期依赖表达和核定位的表征。这些基因的mRNA水平经历细胞周期依赖性振荡，在G1/S期达到峰值，可能受到E2F基序的调节，其中两个在HsMCM5基因的5'上游区域检测到。相反，这些Mcm蛋白的蛋白水平在HeLa细胞周期中保持相当稳定。然而，随着KD细胞从GO期进入G1/S期，它们的水平以不同的方式逐渐增加。在氧化石墨烯阶段，HsMcm2和-5蛋白的数量远低于HsMcm7和-3蛋白的数量，表明它们的化学计量量不存在，并且只有一部分这些分子作为Mcm/P1复合物的一部分积极参与细胞周期调节。
We report here the comparative analysis of human Mcm/P1 proteins (HsMcm2, -3, -5 and -7), including a characterization of their mutual interactions, cell cycle dependent expression and nuclear localization during the cell cycle and the quiescent state. The mRNA levels of these genes, which undergo cell cycle dependent oscillations with a peak at G1/S phase, may be regulated by E2F motifs, two of which were detected in the 5' upstream region of the HsMCM5 gene. In contrast, the protein levels of these Mcm proteins were found to remain rather constant during the HeLa cell cycle. However, their levels gradually increased in a variable manner as KD cells progressed from GO into the G1/S phase. In the GO stage, the amounts of HsMcm2 and -5 proteins were much lower than those of HsMcm7 and -3 proteins, suggesting that they are not present in stoichiometric amounts, and that only a proportion of these molecules actively participate in cell cycle regulation as part of Mcm/P1 complexes.