Conversion of Lactobacillus pentosus D-lactate dehydrogenase to a D-hydroxyisocaproate dehydrogenase through a single amino acid replacement
Conversion of Lactobacillus pentosus D-lactate dehydrogenase to a D-hydroxyisocaproate dehydrogenase through a single amino acid replacement
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DOI：
10.1128/jb.185.16.5023-5026.2003
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发表时间：
2003-08-01
影响因子：
3.2
通讯作者：
Taguchi, H
中科院分区：
文献类型：
--
作者：
Tokuda, C;Ishikura, Y;Taguchi, H
酪氨酸取代酪氨酸后，戊糖乳杆菌依赖NAD的D-乳酸脱氢酶对较大的脂肪族或芳香族2-酮酸底物的活性提高了3-4个数量级，而对丙酮酸的活性降低了约30倍，将该酶转化为高活性的D-2-羟基异己酸脱氢酶。
The single amino acid replacement of Tyr52 with Leu drastically increased the activity of Lactobacillus pentosus NAD-dependent D-lactate dehydrogenase toward larger aliphatic or aromatic 2-ketoacid substrates by 3 or 4 orders of magnitude and decreased the activity toward pyruvate by about 30-fold, converting the enzyme into a highly active D-2-hydroxyisocaproate dehydrogenase.