Suppression of RIZ in biologically unfavourable neuroblastomas
Suppression of RIZ in biologically unfavourable neuroblastomas
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DOI：
10.3892/ijo_00000784
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发表时间：
2010-11-01
影响因子：
5.2
通讯作者：
Larsson, Catharina
中科院分区：
文献类型：
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作者：
Geli, Janos;Kiss, Nimrod;Larsson, Catharina
神经母细胞瘤是一种儿科实体瘤，其特征是具有预后重要性的复发性基因组异常。最常见的异常之一是1号染色体短臂的缺失和该染色体臂中癌症相关基因表达的减少。视网膜母细胞瘤蛋白相互作用锌指基因（RIZI）的长同种型是已知的肿瘤抑制基因和位于1p36.2的成神经细胞瘤候选基因。本研究的目的是进一步评估RIZ可能参与神经母细胞瘤的发展。在一组神经母细胞瘤细胞系和肿瘤（33个神经母细胞瘤和3个神经节神经瘤）中定量RIZ转录物的表达。通过亚硫酸氢盐焦磷酸测序定量驱动RIZ1表达的启动子P1的甲基化状态。只有低水平的启动子甲基化（
Neuroblastoma is a paediatric solid tumor characterized by recurrent genomic abnormalities of prognostic importance. One of the most commonly observed abnormalities is deletion of the short arm of chromosome 1 and reduced expression of cancer related genes in this chromosomal arm. The long isoform of the retinoblastoma protein-interacting zink finger gene (RIZI) is a known tumor suppressor and a candidate neuroblastoma gene located at 1p36.2. The present study was undertaken to further assess the possible involvement of RIZ in neuroblastoma development. Expression of RIZ transcripts were quantified in a panel of neuroblastoma cell lines and tumors (33 neuroblastomas and 3 ganglioneuromas). Methylation status of promoter PI driving RIZ1 expression was quantified by bisulfite Pyrosequencing. Only low mean levels of promoter methylation (