High prevalence of Mycobacterium avium subspecies paratuberculosis IS900 DNA in gut tissues from individuals with Crohn's disease
High prevalence of Mycobacterium avium subspecies paratuberculosis IS900 DNA in gut tissues from individuals with Crohn's disease
复制标题
DOI：
10.1136/gut.2004.045526
复制
发表时间：
2005-07-01
期刊：
影响因子：
24.5
通讯作者：
Schmidt, J
中科院分区：
文献类型：
--
作者：
Autschbach, F;Eisold, S;Schmidt, J
背景和目标：在克罗恩病（CD）组织中存在鸟分枝杆菌副结核亚种（MAP）特异性IS900 DNA的确证结果。因此，我们在CD（n = 100）和溃疡性结肠炎（UC，n = 100）患者的大量肠道样本以及非炎症对照组织（nIBD，n = 100）中检查了IS900。我们假设，IS900 DNA检测可能与不同的临床表型特征CD。方法：使用机械酶破坏技术和巢式IS900特异性聚合酶链反应（PCR）检查手术切除的组织中MAP DNA的患病率。结果：CD组织标本中IS900 PCR检出率（52%）明显高于UC（2%）和nIBD（5%）（p < 0.0001）。在CD患者中，在患病小肠（47%）和结肠（61%）的样本中检测到IS900 DNA。MAP特异性IS900检测率与CD的临床表型特征之间没有确定的相关性。结论：MAP特异性IS900 DNA的存在是CD的主要特征。针对MAP的治疗性干预可能是克罗恩病缓解的潜在靶点。
Background and aims: Conflicting results exist about the presence of Mycobacterium avium subspecies paratuberculosis (MAP) specific IS900 DNA in Crohn's disease (CD) tissues. Therefore, we examined IS900 in a large number of gut samples from patients with CD (n = 100) and ulcerative colitis (UC, n = 100), and in non-inflamed control tissues (nIBD, n = 100). We hypothesised that IS900 DNA detection might be associated with distinct clinical phenotypic characteristics in CD.Methods: The prevalence of MAP DNA in surgically resected tissues was examined using a mechanical-enzymatic disruption technique and nested IS900 specific polymerase chain reaction (PCR). CD patients were stratified according to the criteria of the Vienna classification and other clinical characteristics.Results: IS900 PCR detection rate was significantly higher in CD tissue samples (52%) than in UC (2%) or nIBD (5%) specimens (p < 0.0001). In CD patients, IS900 DNA was detected in samples from both diseased small bowel (47%) as well as from the colon (61%). No firm association between MAP specific IS900 detection rates and clinical phenotypic characteristics in CD could be established. However, corticosteroid medication constituted a factor which tended to have a negative influence on IS900 DNA detection rates in CD (p < 0.01).Conclusions: The presence of MAP specific IS900 DNA is a predominant feature of CD. Therapeutic intervention against MAP might represent a potential target for disease mitigation in Crohn's disease.