NUCLEOTIDE-SEQUENCES THAT SIGNAL THE INITIATION OF TRANSCRIPTION AND TRANSLATION IN BACILLUS-SUBTILIS
NUCLEOTIDE-SEQUENCES THAT SIGNAL THE INITIATION OF TRANSCRIPTION AND TRANSLATION IN BACILLUS-SUBTILIS
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DOI：
10.1007/bf00729452
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
LOSICK, R
中科院分区：
文献类型：
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作者：
MORAN, CP;LANG, N;LOSICK, R
测定了在营养细胞中发现的枯草杆菌RNA聚合酶(β55-RNA聚合酶)所利用的两个枯草杆菌启动子(VEG和TMS)的核苷酸序列，并将其序列与以前报道的几个芽孢杆菌启动子的序列进行了比较。以VEG和TMS转录起始点上游约35个碱基对(“-35”区)和10个碱基对(“-10”区)为中心的六核苷酸序列(被17个碱基对隔开)与属于大肠杆菌启动子的共识六核苷酸(TTGACA和TATAAT)密切对应。然而，与优选的-35和-10序列的一致性可能不足以促进枯草杆菌RNA聚合酶的有效利用，因为枯草杆菌RNA聚合酶以非常不同的效率使用了3个符合这些序列并被E.coliRNA聚合酶有效利用的启动子(VEG、TMS和E.colitac)。对位于8个枯草杆菌染色体和噬菌体启动子下游的DNA中的mRNA序列进行了分析，以寻找可能发出翻译启动信号的核苷酸序列。按照McLaughlin等人的规则。艾尔(1981)，发现了与枯草杆菌16S rRNA 3‘’末端具有广泛互补性的m RNA核苷酸序列，随后观察到一个起始密码子和一个开放阅读框架。
The nucleotide sequences of 2 B. subtilis promoters (veg and tms) that are utilized by the principal form of B. subtilis RNA polymerase found in vegetative cells (.sigma.55-RNA polymerase) were determined, and the sequences were compared to those of several previously reported Bacillus promoters. Hexanucleotide sequences centered approximately 35 (the "-35" region) and 10 (the "-10" region) base pairs upstream from the veg and tms transcription startpoints (and separated by 17 base pairs) corresponded closely to the consensus hexanucleotides (TTGACA and TATAAT) attributed to Escherichia coli promoters. Conformity to the preferred -35 and -10 sequences may not be sufficient to promote efficient utilization by B. subtilis RNA polymerase, however, since 3 promoters (veg, tms and E. coli tac) that conform to these sequences and that are utilized efficiently by E. coli RNA polymerase were used with highly varied efficiencies by B. subtilis RNA polymerase. mRNA sequences in DNA located downstream from 8 B. subtilis chromosomal and phage promoters were analyzed for nucleotide sequences that might signal the initiation of translation. In accordance with the rules of McLaughlin et. al. (1981), mRNA nucleotide sequences were observed with extensive complementarity to the 3'' terminal region of B. subtilis 16S rRNA, followed by an initiation codon and an open reading frame, were observed.