The effect of sampling time and sample handling on the detection of Staphylococcus aureus in milk from quarters with subclinical mastitis.
The effect of sampling time and sample handling on the detection of Staphylococcus aureus in milk from quarters with subclinical mastitis.
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采样时间和样品处理对亚临床乳腺炎患者牛奶中金黄色葡萄球菌检测的影响。
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
D. Kelton
中科院分区：
文献类型：
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作者：
S. Godden;J. Jansen;K. Leslie;N. Smart;D. Kelton
金黄色葡萄球菌乳腺炎是造成乳品行业经济损失的重要原因。控制计划依赖于及时、准确地识别阳性区域。检查了采样时间和样品处理的影响，试图提高金黄色葡萄球菌检测的准确性。从 55 个患有亚临床金黄色葡萄球菌感染的哺乳期采集了挤奶前和挤奶后的牛奶样本。每个样品被分成2等份；其中一份是新鲜培养的，另一份是-20℃冷冻14天后培养的。方差分析用于确定采样时间（挤奶前与挤奶后）和样品处理（新鲜与冷冻）对金黄色葡萄球菌检测的影响，通过每毫升菌落形成单位的平均类别 (cfu/mL) 进行测量。由于采样时间和样品处理之间的相互作用，需要进行分层分析。只有新鲜的挤奶后样品较差，产生的 cfu/mL 平均类别较低（P < 0.05）。在新鲜或冷冻的挤奶前样品以及冷冻的挤奶后样品中，通过 cfu/mL 的平均类别测量，检测亚临床乳房内感染的金黄色葡萄球菌的能力达到最大。
Staphylococcus aureus mastitis is an important cause of economic loss for the dairy industry. Control programs rely on the timely and accurate identification of positive quarters. The effects of sampling time and sample handling were examined in an attempt to improve the accuracy of detection of S. aureus. Premilking and postmilking milk samples were collected from 55 lactating quarters with subclinical S. aureus infection. Each sample was divided into 2 aliquots; one of which was cultured fresh, the other was frozen at -20 degrees C for 14 days before being cultured. Analysis of variance was used to determine the effect of sampling time (premilking vs postmilking) and sample handling (fresh vs frozen) on the detection of S. aureus, as measured by the mean category for colony-forming units per millilitre (cfu/mL). A stratified analysis was required, due to interaction between sampling time and sample handling. Only a fresh postmilking sample was inferior, yielding a lower mean category for cfu/mL (P < 0.05). The ability to detect S. aureus in quarters with subclinical intramammary infection, as measured by the mean category of cfu/mL, was maximized in fresh or frozen premilking samples and in frozen postmilking samples.