Expression of the gene encoding protein A in Staphylococcus aureus and coagulase-negative staphylococci
Expression of the gene encoding protein A in Staphylococcus aureus and coagulase-negative staphylococci
复制标题
编码蛋白A的基因在金黄色葡萄球菌和凝固酶阴性葡萄球菌中的表达
作者：
M. Uhlén;B. Guss;B. Nilsson;F. Götz;M. Lindberg
构建了两个含有金黄色葡萄球菌A蛋白基因(SpA)的穿梭载体，以研究该基因在不同的金黄色葡萄球菌以及凝固酶阴性的表皮葡萄球菌、头葡萄球菌和木糖葡萄球菌中的表达。一种名为pSPA15的质粒含有A蛋白的完整结构基因，在各种葡萄球菌中，A蛋白与细胞壁结合。另一个质粒pSPA16编码一个截短的蛋白A，缺少C-末端部分，称为X区。X区在所有被测试的葡萄球菌中都是胞外的，这证实了X区对细胞壁结合的重要性。质粒编码蛋白A在不同菌株中的表达与染色体spA基因的表达密切相关。在金黄色葡萄球菌Cowan I和A676菌株中，凝固酶阴性物种表达质粒编码的蛋白A产生染色体spA基因编码量的12%至30%。
Two shuttle vectors containing the gene for protein A (spa) from Staphylococcus aureus have been constructed to study expression of the gene in various strains of S. aureus and in the coagulase-negative species Staphylococcus epidermidis, Staphylococcus capitis, and Staphylococcus xylosus. One plasmid, pSPA15, contains the complete structural gene for protein A, which binds to the cell wall in various Staphylococcus species. The other plasmid, pSPA16, codes for a truncated protein A lacking the C-terminal part called region X. The latter is exclusively extracellular in all Staphylococcus species tested, which confirms the importance of region X for cell wall binding. The expression of the plasmid-coded protein A in various strains of S. aureus is strongly correlated to the expression of the chromosomal spa gene. The coagulase-negative species expressing plasmid-encoded protein A produce 12 to 30% of the amount coded by the chromosomal spa gene in S. aureus strains Cowan I and A676.