Cartilage tolerates single impact loads of as much as half the joint fracture threshold
Cartilage tolerates single impact loads of as much as half the joint fracture threshold
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DOI：
10.1097/01.blo.0000136653.48752.7c
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发表时间：
2004-09-01
影响因子：
4.2
通讯作者：
Silva, MJ
中科院分区：
文献类型：
--
作者：
Borrelli, J;Zhu, Y;Silva, MJ
我们假设一次机械损伤可以影响细胞增殖、基质周转和软骨的结构完整性，并且这些影响将是剂量依赖性和时间依赖性的。使用先前确认的摆锤器械，对新西兰白色家兔的内侧股骨髁后部施加低冲击（14.4 MPa +/- 2.1 MPa）、中等冲击（22.8 MPa +/- 5.8 MPa）或高冲击（55.5 MPa +/- 12.6 MPa）的冲击载荷。在撞击后2、6和12周对动物实施安乐死，并收获撞击和假（对侧肢体）软骨。每个标本进行了评估，通过光学显微镜和免疫组化方法。虽然影响标本有更大的损失蛋白多糖染色比假软骨，这些变化既不依赖于剂量，也不依赖于时间。在不同影响组中未发现结构损伤、酶促蛋白聚糖或胶原分解或细胞增殖。关节软骨是一种弹性组织，特别是在原位，可以承受高达45%的关节骨折阈值的单次冲击载荷，而不会出现相当大的破坏或退化。
We hypothesized that one mechanical insult could affect cellular proliferation, matrix turnover, and the structural integrity of cartilage, and that these effects would be dose dependent and time dependent. One impact load of low impact (14.4 MPa +/- 2.1 MPa), medium impact (22.8 MPa +/- 5.8 MPa), or high impact (55.5 MPa +/- 12.6 MPa) was administered to the posterior aspect of the medial femoral condyle of New Zealand White rabbits using a previously validated pendulum device. Animals were euthanized at 2, 6, and 12 weeks after impact, and the impacted and sham (contralateral limb) cartilage were harvested. Each specimen was assessed by light microscopy and by immunohistochemical methods. Although impacted specimens had greater loss of proteoglycan staining than sham cartilage, these changes were neither dose dependent nor time dependent. No structural damage, enzymatic proteoglycan or collagen breakdown, or cellular proliferation was identified in the different impact groups. Articular cartilage is a resilient tissue, particularly in situ, and can tolerate single impact loads of as much as 45% of the joint fracture threshold without considerable disruption or degradation.