Neuraminidase is important for the initiation of influenza virus infection in human airway epithelium
Neuraminidase is important for the initiation of influenza virus infection in human airway epithelium
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DOI：
10.1128/jvi.78.22.12665-12667.2004
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发表时间：
2004-11-01
影响因子：
5.4
通讯作者：
Klenk, HD
中科院分区：
文献类型：
--
作者：
Matrosovich, MN;Matrosovich, TY;Klenk, HD
流感病毒神经氨酸酶（NA）在病毒复制过程中，对病毒粒子的释放和传播起着重要作用。为了测试NA是否也能促进病毒进入细胞，我们在NA抑制剂奥司他韦羧酸盐存在下，用人和禽流感病毒感染人气道上皮细胞培养物。在病毒应用后7小时，药物处理的培养物中感染的细胞比未处理的培养物少20至500倍（P < 0.0001），表明药物抑制了感染的开始。这些数据表明，病毒NA在感染早期起作用，它们为预防性使用NA抑制剂提供了进一步的理论基础。
Influenza virus neuraminidase (NA) plays an essential role in release and spread of progeny virions, following the intracellular viral replication cycle. To test whether NA could also facilitate virus entry into cell, we infected cultures of human airway epithelium with human and avian influenza viruses in the presence of the NA inhibitor oseltamivir carboxylate. Twenty- to 500-fold less cells became infected in drug-treated versus nontreated cultures (P < 0.0001) 7 h after virus application, indicating that the drug suppressed the initiation of infection. These data demonstrate that viral NA plays a role early in infection, and they provide further rationale for the prophylactic use of NA inhibitors.